(at around 1h 26 mins) After falling in ice cold water, Owen is barely wet in the lift, nor is he shivering, which he would be, purely from the shock.
(at around 1h 40 mins) When the locust swarm is let loose on the Biosyn field, the swarm is much larger than what could have fit inside the cell that had previously contained it. Significant space within that cell was dedicated to locusts in various states of development, so not all of them would even have been able to fly as shown.
(at around 1h 23 mins) Claire goes underwater to evade a dinosaur but after that, her hair\clothes\etc look as if it never happened.
(at around 1h 28 mins) The security man tells Dodgson that the cameras saw Ian Malcolm put something in Ellie's pocket, which he believes was Malcolm's security key. Malcolm handed his key bracelet to Ellie (at around 42 mins), he did not put it into her pocket.
When Owen, Claire, and Maisie leave their cabin in Nevada, the weather is frigid and the whole area is covered in snow. When they return Beta to Blue just a few days later, the area is not only thawed but covered in greenery, which would be impossible in such a short time.
(at around 1h 2 mins) Raptors in the Jurassic Park series are established as being capable of running 40-60 mph. However, when Owen, who is on a motorcycle, is being chased by two raptors, he escapes by running onto the ramp of a cargo plane that is taking off. In order to get sufficient life, larger planes need to go over 100 mph. The raptors would have been left far behind, but one gets onboard (briefly).
(at around 57 mins) Raptors in the Jurassic Park series are established as being capable of running 40-60 mph. An average human can sprint at less than a quarter of that speed, yet Claire is somehow able to outrun a raptor for several minutes.
(at around 1h 24 mins) After the plane crash when Kayla and Owen are walking on the ice, it is cracking under their weight. The plane is in the background broken through the ice and the broken ice chunks are seen to be at least 4" (10 cm) thick. Ice that thick would easily bear the weight of a person.
(at around 1h 40 mins) When the giant locusts caught fire, it would have burned their more delicate wings off first, rendering them unable to swarm up in the sky.
(at around 56 mins) When Claire confronts Santos, Claire zaps her with a hand-held shocking device which sends Santos flying across the room. An electric shock will not exert that amount of force regardless of its voltage.
Dinosaurs were thought to be cold-blooded for many years. Had that been true Blue and her offspring would not have fared well in the frigid climate they are shown inhabiting. However, newer research, including a landmark May 2022 paper in Nature, has proven that the vast majority of dinosaurs (including velociraptors like Blue) were warm-blooded.
(at around 1h 24 mins) For cold weather with snow on the ground, you don't see their breath and they walk around wearing only a shirt and show no sign of it being cold.
(at around 43 mins) When Lewis is in the lab with locusts, there is a Surface Studio computer on the desk, the power cord at the back is missing, meaning the computer is not functional at all.
(at around 17 mins) Maisie looks at the album, the first photo is Maisie and the second is Maisie with Iris, you can see Maisie's face is the same the same way. It's obviously photoshop.
(at around 3 mins, extended version only) The prologue, set in the Cretaceous, depicts a Giganotosaurus fighting with and killing a Tyrannosaurus (who turns out to be the genetic blueprint for Rexy). Both creatures lived in separate continents and in different time periods. There were not even similar creatures to either that coexisted. The relatives of Giganotosaurus (and Allosaurus) went extinct in North America before the ancestors of Tyrannosaurs (which were the size of a raptor at that time) were able to grow into Tyrannosaurus-like size.
(at around 1h 20 mins) There is no tropical forest located in the Dolomites.
(at around 2h 10 mins) The Italian mountain rescue helicopters are colored yellow and red, not white and red as depicted toward the end of the movie. Austrian rescue helicopter, still plausible given the location, would be yellow instead.
(at around 24 mins) Alan said that the BioSyn Sanctuary in the Dolomites is at 100 miles from everything. The Alps are pretty densely populated and medium sized cities like Bolzano and Innsbruck are well inside that distance. To even find a single valley without human settlements would be hard.
(at around 1h 50 mins) The observation station that the human cast escapes to has large windows that the Giganotosaurus is easily able to break. Such an outpost should have smaller windows with sturdy dividers placed close enough together that the dinosaurs they'd want to observe could not fit between them.
(at around 35 mins) When Claire and Ellie are heading to redistribute the power, they turn a corner and are surprised by the sight of a large number of burning locusts. Any burning animal matter would give off a very strong odor, so it should not take until they nearly step on them to notice there are burning bugs nearby.
(at around 1h 35 mins) The Dimetrodon lived during the Permian period which didn't have mosquitoes, so they couldn't possibly have been bitten by mosquitoes, which couldn't have been stuck in amber, and then used to retrieve the Dimetrodon DNA to recreate the lost species.
(at around 1h 50 mins) In Lewis Dodgson's office, the Barbasol shaving cream can which he gave to Dennis Nedry in Jurassic Park (1993) can be seen. However in that movie when Nedry was killed by a Dilophosaurus, the can got washed away in a canal in Isla Nublar, where Dodgson could not have retrieved it from because he wouldn't know its exact location as the can could be anywhere in the island.
During the Dimetrodon attack, when Malcolm tries to open the gate, he says he doesn't have the code, and that there are 10,000 possibilities. Except 10,000 possibilities means it's a 4-digit code, and he has no way of knowing how many digits long the code is.
(at around 1h 9 mins) Henry Wu believes that if he can understand how Maisie's mother fixed her genetic condition then he can create a similar procedure to wipe out the locusts that BioSyn unleashed on the world. He is fully aware that Maisie was treated via a series of direct injections. Creating a process that could be passed from locust to locust like a pathogen would be vastly different.
(at around 55 mins) When confronted by Atrociraptors, none of the team of agents, all of whom are armed with guns, attempt to shoot the attacking dinosaurs.
(at around 1h 30 mins) When the Giganotosaurus confronts the T-rex, Owen asks if it's an Allosaurus. Allosaurus was significantly smaller than the T-rex, while the Giganotosaurus is noticeably larger. Owen was an employee at Jurassic World, he should know these basic facts about dinosaurs.
(at around 40 mins) When trying to impart on Ian Malcolm how dire the situation is, Ellie tells him that it is "seven minutes to midnight," a reference to the Doomsday Clock. On the Doomsday Clock, seven minutes to midnight is not overly serious. That was where the clock was originally set upon its creation in 1947. For comparison, the clock was set at two minutes to midnight in 2018, and at just 100 seconds to midnight in 2020, where it remained during the course of this film's production and release.
(at around 1h 24 mins) After leaving the crashed plane, Owen and Kayla walk across the ice towards the cliff face, and are subsequently attacked by a dinosaur. They should have just walked the other direction towards the damn (which was much closer) and used its walkway to get off the ice, which is what they end up doing anyway when they run away from the attacking dinosaur.