The names X-Ray and Vav come from a Achievement Hunter video which Gavin and Ray were in.
In this video, Gavin accidently calls himself "Vav" instead of "Gav".Later in the same video, Gavin jokingly asks Ray if anyone has ever nicknamed him "X-Ray".
This became a running gag between them until Gavin made a joke in another video about how X-Ray and Vav could be crime fighters and the Rooster Teeth community loved the idea.
Two of the enemies in the first two episodes are based on creations from achievement hunter videos, just like X-Ray and Vav. The Corpirate comes from one of Geoff's creations and is voiced by Geoff in the series. The Mad King is in reference to Ryan's personality and evilness towards cows.
Ash Samaya is Indian (Lindsay's mentioned that a bunch: in the Afterbuzz interviews and the DVD commentary and in the Chaos Corner she did with Elyse) and she is named after Bollywood star Aishwarya (cause Lindsay loves Bollywood, but Jordan said the name was too long so that's why its just Ash but Ash is short for Aishwarya)