(at around 1 min) At the beginning, Ego is running down through the woods with Meredith, in a split second long shot she is wearing Ugg Boots, however, in the later shots of her feet, she is wearing a pair of heels.
(at around 1h 11 mins) When Peter Quill is talking to Gamora on Ego, he tosses a radio on the chair. A moment later the radio's position on the chair shifts further to the left, then back again.
(at around 55 mins) When Ego is showing Quill how to create the ball of energy, he places Quill's hands with palms facing each other. The camera angle changes and Quill's hands are facing up.
(at around 1h 11 mins) Peter Quill typically wears his collapsed helmet, a shiny metallic plate-like object, behind his right ear at all times, including when he is in casual attire. However, when he is dancing with Gamora on Ego's Planet, he is markedly without it. In all other scenes on the planet, before and after, he is wearing it.
(at around 10 mins) When Quill tells the High Priestess he doesn't like "anything casual," he starts to clasp his hands together. In the next shot, they are at his sides.
People exposed to the vacuum of space do not freeze in a matter of seconds/minutes. In a vacuum the only way to lose (or gain) body heat is through thermal radiation. It would take hours and hours. This is what makes a vacuum a good insulator. In fact, if you were to float in sunlight you'd sooner cook than freeze.
Correction: The pressure is important: Your eyes, if left open, would basically boil and surface freeze because of lack of pressure on the liquid. Your tongue would be susceptible as well. The pressure issue would also make it impossible to hold air in your lungs, so you would pass out within a minute, but likely much sooner: "In 1965, while performing tests at the Johnson Space Center, a subject was accidentally exposed to a near vacuum (less than 1 psi) when his space suit leaked while in a vacuum chamber.
He did not pass out for about 14 seconds, by which time unoxygenated blood had reached his brain. Technicians began to repressurize the chamber within 15 seconds and he regained consciousness at around the equivalent of 15,000 feet of altitude. He later said that his last conscious memory was of the water on his tongue beginning to boil. So, there's at least one data point about what it's like to be in a vacuum. It won't be pleasant, but it won't be like the movies, either."
Correction: The pressure is important: Your eyes, if left open, would basically boil and surface freeze because of lack of pressure on the liquid. Your tongue would be susceptible as well. The pressure issue would also make it impossible to hold air in your lungs, so you would pass out within a minute, but likely much sooner: "In 1965, while performing tests at the Johnson Space Center, a subject was accidentally exposed to a near vacuum (less than 1 psi) when his space suit leaked while in a vacuum chamber.
He did not pass out for about 14 seconds, by which time unoxygenated blood had reached his brain. Technicians began to repressurize the chamber within 15 seconds and he regained consciousness at around the equivalent of 15,000 feet of altitude. He later said that his last conscious memory was of the water on his tongue beginning to boil. So, there's at least one data point about what it's like to be in a vacuum. It won't be pleasant, but it won't be like the movies, either."
(at around 46 mins) Ego tells Quill that he wanted to experience what it was to be human-- but at the point in time he's describing, he had not yet encountered any other life. (In point of fact, humans aren't even the first species he discovers or mates with). However, at that time, Ego was trying to gain Quill's empathy before telling him the truth - he used "humans" since his son was half human, but was lying.
(at around 21 mins) When Peter's ship is crashing into the forest on Berhert, Drax is still outside, being pulled along on a tether. When the ship hits the trees, it slows down. If Drax's speed remains constant, he should be pulled closer to the ship, perhaps even crashing into the cargo hold, yet the tether remains fully stretched (so much so that it eventually breaks off). This is because Drax also slows down by hitting the trees himself and due to air resistance.
(at around 2h) Unlike the iconic Walkman and headphones, the headphones used with the Zune are not original Zune headphones. However with all MP3 players the original owner of the Zune could've broken or swapped out the original headphones for the ones that Quill is using at the time.
The trailer dialogue between Star Lord and Gamora about her holding a rifle is different from the final release, but it should be noted that some scenes in a trailer could be changed or deleted in the final cut.
At the end of the movie, (Yondu's funeral is still kind of going on) Gamora's green skin makeup is not completely covering the area around her eyes.
(at around 39 mins) During the ambush scene on Berhert, when Rocket is jumping from one man's shoulder to another, the same rendering of Rocket appears in two consecutive frames in this fast-moving shot.
Star Lord's father should had turned into a younger David Hasselhof, since he had no way of knowing how older David Hasselhof would look like.
Peter says that he pretended his father was David Hasselhoff and that he was either away shooting films or touring Germany with his band. While Hasselhoff had 2 albums in the mid-80ies he became successful and known as a singer after Peter's abduction when "I've been looking for freedom" topped the German charts in 1989. Peter shouldn't be aware of Hasselhoffs Music career or his success in Germany.
(at about 1:40) Baby Groot yells "Weeeee." Rocket has explained that he can only say I. Am. and Groot. Exclusively in that order.