Unlike the comic version, the Pluffs do not have red eyes.
Also unlike the comic version, the Pluffs are evil counterparts of the Smurfs from an alternate universe, while in the comic, they were evil darker toned duplicates of the Smurfs magically created by Papa Smurf.
This episode is based on the "mirror universe" concept created by the episode "Mirror, Mirror" from the original 1960s Star Trek TV series. It also adapts elements from the comic book story "The Smurf Menace".
The travel between universes using Brainy's two-wheeled cycle is similar to how the DeLorean time machine travels through time in the Back To The Future franchise, right down to the flaming tire tracks being left behind.