The zipping sound effect that is heard when Mario is sucked up the pipe was later used in Super Mario 64 (1996).
The animated cutscenes (alongside those featured in CD-i games Zelda: The Wand of Gamelon (1993) and Link: The Faces of Evil (1993)) have become internet memes in the mid-2000s, featured in several spoof/remix videos uploaded to YouTube.com, known as "YouTube Poops".
After the release of this game, Philips intended to release a sequel to Super Mario World (1990) called "Super Mario's Wacky World's". The game was cancelled, but a pre-alpha prototype later surfaced on the internet.