When Alexander Anderson enters the museum and starts walking down the hallway, the number of blades he is holding in his left hand changes. It is first seen that he has four, then one, then three.
During the Alucard and Tubalcain Alhambra (Dandy Man) fight after Alucard has broken Tubalcain Alhambra's leg Alhambra goes to slash at Aluacard with the Ace of Spades between his fingers in response to Alucard doing the same with his open hand. However, just before the hand and card meet Tubalcain Alhambra's playing card is no longer the Ace of Spades and is in fact the Ace of Diamonds but when Alucard splits the card in two it is in fact the Ace of Spades again.