When House is talking with Chase and Thirteen, the camera pans past some objects on his desk, one of which is a picture of Stephen Colbert (with a magnifying glass in front of it). Colbert has a picture of House on the mantle on the set of The Colbert Report (2005).
About 39:24, House refers to "little deaths." In French, an orgasm is referred to as "la petite mort," which means "the little death."
House wonders if the patient's husband is a "plushie." A plushie is someone interested in stuffed animals. In some cases this is manifested as a sexual attraction, although this is more the exception than the norm.
A barium enema is not performed with the patient lying on the side as shown; it is done lying on the stomach with left leg bent (known as the Sims position).