After the title sequence while Ted tells his friends about Heather's several youthful follies the extra in the blue shirt stands up for an approaching woman. After a cut on Lily and Marshall the guy is sitting and the woman stands at the table.
When Robin is retelling Marshall's story at the bar, she is covering her heart with her left hand when she says, "A little part of me just died, son." When the camera looks back at her after she's asked to clarify, she's covering her heart with her right hand.
Throughout the show Marshall takes great pride in the fact that people from Minnesota are warm, friendly and accepting of all. The behavior of people in the bar is contrary to this.