When Peter gets up to go to the interrogation room to speak with Dr. Steig, the video display in the background shows Peter stepping in to the room before he gets anywhere near it.
During the introduction scene of Olivia and John, Olivia takes a call on her cell phone, and gets out of bed to dress whilst the camera stays on John. In the seven seconds of screen time that follows, during the same conversation in real time, apart from having her shirt to button up, Olivia is now fully dressed and opens the door to leave for work.
When Olivia chases John along the highways both the amount of snow falling from the sky as well as the amount on the ground changes from shot to shot.
After Agent Scott suffocates Steig with a pillow, he pulls it off of his face. An agent that Dunham had instructed to check on Steig and Scott at the hospital who arrived afterwards is seen pulling the pillow off of Steig's face.
During the final car chase the vehicle headlights are alternately on and off.
Walter requests a 2 year old mature pure-bred cow weighing 850 pounds, however a mature 2 year old Holstein cow weighs an average of 1,200 pounds. Anything less than 1,000 pounds at 2 years old would be severely malnourished.
Dr Bishop asks for a couple of cows in his lab. When asked why, his son Peter interjects it's because of how similar they are to humans with only a couple of lines of genetic code being different. Cows share only ~80% of a human's DNA which is still the reason the right type of mice are used. The fact a human shares ~99.9% with another human means that small difference alone equates to ~500 pages of a book of genetic code.
Broyles specifies that anyone entering the plane must wear a level 4 Hazmat suit. Hazmat suits are graded from level A, the most protective down to level D, the least protective. Broyles was possibly referring to laboratory-based Biosafety level standards which are reversed in order such that BSL 4 is the most impervious.
Charlie makes reference to a tip from "Back Bay PD." The Back Bay is a Boston neighborhood but does not have its own police force and is nowhere near Chelsea, where the tip directed them to.
When they show Iraq for the first time, it's shown that two Iraqi guys speak Farsi to each other (with a very weird accent) and the American guy tells them "I also know Farsi". While Farsi is the language spoken in Iran, Arabic is spoken in Iraq. However, later episodes explain that Peter was meeting with Iranian businessmen.
The German spoken by the passengers and crew of the airplane in the beginning is sometimes grammatically incorrect, sometimes nonsensical, and often tinted with a heavy American accent.
When Peter is speaking to the two Iraqi men regarding the pipeline, after stating that he speaks Farsi, the shot of the two men ceasing their conversation and shifting in their seats is obviously the previous shot of them beginning their conversation, except reverse frame order.
The show is supposed to take place in Boston; however the ambulance taking John Scott to Walter's laboratory has New York license plates.
Towards the end when Olivia opens her car door to pursue John, a male crew member is reflected in the window. When the pursuit has ended and she runs out of the car, a reflection of the moving camera can be seen in the car's bodywork.
The pilot is supposed to be set in Boston yet the geography is clearly not that of Boston and every shot which shows the skyline is clearly that of another city.
On the plane, the pilots and the stewardess speak German. All communication aboard aircraft has to be in English.