This marks the debut of Cooler, the optimistic leader with a witty sense of humor and wears his trademark blue leather-jacket; The Nose(Later renamed Nose Marie in the TV Series), a girl dog who wears a blue skirt with the remarkable sense of smell; Howler, the inventive dog with curly, yellow hair who only howls and wears a red derby; and Bright Eyes, another girl dog who has brown hair and blue eyes. They will appear again in their own TV Series.
Tubbs and Flack were inspired by Horace and Jasper from Disney's One Hundred and One Dalmations respectively.
Scrounger, Violet Vanderfeller, and Barkerville later made a cameo appearance in the beginning of Cooler's flashback in Wagga Wagga. Scrounger and Barkerville made another cameo appearance in The Captain and the Cats. Scrounger was the only character to make a guest starring appearance in Garbage Night the Musical.
Mayor Fist later made a guest appearance as one of the judges of the Annual Pet Talent Show in Bright Lights, Bright eyes/Dog and Caterpillar.
Dr. Weston was remade twice in the Pound Puppies TV Series. In Snowbound Pound, she was remade as an Asian-American veterinarian. In Season 2 of the Pound Puppies TV Series, she was remade as an African-American veterinarian, Dr. Simon.