Just before the infected come over the wall in Jerusalem, Gerry says, "If I could get into India, where would I start?" "India's a black hole." The lines originally named Russia, not India, and you can still read Russia on his lips. The editing of the scene fails to completely conceal the second overdub as well.
At the beginning of the film, in the traffic jam, a motorcycle policeman pulls up and tells Gerry to get back in his car; there is nothing behind him but gridlocked traffic; a second later when he tells Gerry to remain in his vehicle, he's suddenly mown down by a PWD dump truck even though as he's pulling up there's two lanes of cars and taxis backed up to the end of the street with no dump truck in sight and no way for it to charge through unobstructed.
When the motorcycle hits the side mirror of Gerry's car in the traffic jam, the mirror falls between Gerry's car and the vehicle in front of him. When Gerry picks up the mirror, it is already in the front wheel of his car.
In the opening action scene the zombies looks super aggressive when they see a live host that it would breaks through the car windshield. Later when Gerry is trapped in the vault 139, the zombie outside patiently waits without even attempting to break in.
When Gerry and Segen leave the W.H.O center in Wales, Segen is wearing a black hood over her head. In the next scene, immediately after stepping through that gate, Segen's head not covered up by that black hood.
As the passenger plane comes into land at Cardiff Airport the background images show Snowdon and other mountains in the Snowdonia region. It would be highly unlikely that a route from Jerusalem to South Wales would take them to North Wales.
Jerusalem Atarot Airport has long been closed and not used for commercial airline traffic. The only major airport used often for commercial airline traffic, is Tel Aviv Ben Gurion International Airport.
The explosive decompression caused by a large hole suddenly occurring in plane's fuselage takes but a fraction of a second. After that, inside and outside pressures are equal and nothing is sucked out any more, at least not from a distance of several meters from the hole (although outside the hole there is a 900 km/h air stream which is bound to cause some turbulence inside.)
When the grenade detonates in the Airbus, the plane suffers decompression and the pilots' oxygen masks drop, yet the aircraft is flying low (circa 3,000 feet) where the pressure would be equal inside and outside the aircraft so that the oxygen masks would not drop.
When they are done refueling the plane in Korea, the navy seal pulls the hose off like it is a snap on, when in actuality it is a D-1 nozzle which has a lock that has to be turned and then the nozzle itself has to be turned counter clockwise before being taken off.
When arriving in Israel, Gerry Lane uses the call sign 'Reach 394' for his aircraft. While 'Reach' is a real US Air Force call sign, the aircraft in the film is depicted as US Navy and would generally use 'Convoy' as its call sign.
When Lane leaves his family behind on the aircraft carrier he departs on a plane marked US Navy. The plane also has McGuire on the tail fin denoting that it is a USAF plane stationed at Joint Base McGuire in New Jersey.
On passenger aircraft such as the Airbus A310 the protagonist boards in Jerusalem, ladders do not automatically extend from access hatches. The hatches have to be opened (and the ladders lowered) manually.
All throughout the story the Zombies are refer to as being "Dead" but one of the W.H.O. Doctors said that only a living host can carry a virus not a dead one, by this logic the Zombies should not even exist in the first place.
Bacterial pathogens (and even safe bacteria) are stored short-term on agar plates or agar tubes (which are a gel, not an injectible liquid form) in refrigerators. Long-term storage is done in liquid nitrogen. Gerry seems to take the highly pathogenic bacteria from a room temperature clear plastic box. He injects himself with a clear brown liquid, which is not a form that would be in storage. Later, it is announced that people were being injected with a vaccine, which is not a bacteria. At most, it is an attenuated strain of a pathogen, not a living bug. Vaccines do not cause the illness they are meant to protect against.
The helicopter that extracts the family from the Newark rooftop is an HH-65 Dauphin. The U.S. Navy doesn't operate any HH-65 Dauphin helicopters. The U.S. Coast Guard is the only branch of the U.S. military to do so.
When Gerry Lane flies to South Korea, it is shown that he was flying with Antonov An-12 that take-off from Aircraft Carrier. In the real life it is impossible for an airplane as big as Antonov An-12 to conduct take-off from Aircraft Carrier.
Although there are many helicopters patrolling the area around Jerusalem, the pile of zombies that overrun the wall are, for some reason, not noticed by any of them until it was too late to curb the threat.
While flying from South Korea to Jerusalem, there is what appears to be a nuclear explosion. The satellite phone then dies, presumably from the EMP from the explosion. The phone then works later while on plane leaving Jerusalem. It was possible that the phone was fixed while in Jerusalem, but not likely.
During the movie's opening action sequence, British-style traffic lights can be seen at the road junctions, behind the US-style traffic lights, giving away the fact that this scene was filmed in Glasgow, Scotland.
When the Lane family's Volvo is hit by the ambulance at the beginning of the movie, the car is then shown from the driver's side. It is clear that there is no one in the Volvo, and the person driving the ambulance is wearing stunt gear, complete with helmet.
Despite the fact that Gerry (Brad Pitt) is left in a hanging-forward position when his plane crashes, blood on both sides of his face flows straight downward as though he was standing erect after receiving the wounds.
When Gerry leaves for Korea with the Doctor and Navy seals, the doctor is speaking about the virus. There's a shot back to the boat and you see the doctor in the briefing with the group from the first time he speaks about Spanish flu.
The goof items below may give away important plot points.
After the grenade explodes in the plane, the pilots put on blue emergency helmets, equipped with oxygen masks. In the following sequence when they appear, they just wear their standard microphone helmets.
Shortly after the wheels of the Belarus airbus are off the Jerusalem runway, you can clearly see its rear cargo hatch open. An open hatch can not go unnoticed on such a plane. The cargo and passenger compartments are connected so they always have the same pressure for reasons of structural integrity. So with an open hatch, cabin pressurization must fail. Even if there was no cockpit alarm for the open hatch, and nobody wondered about the massive vibrations generated by the hatch as the plane accelerated to speeds above 800 km/h, the oxygen masks would drop long before the plane reached anything near cruising altitude. If a return was impossible like in the zombie scenario, the plane would then have had to stay at a low altitude to avoid all aboard dying from hypoxia, and have used much more fuel, severely restricting range.