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Cameo: Sgt. Fury and his Howling Commandos (Nick Fury, Timothy "Dum Dum" Dugan, Robert "Rebel" Ralston, Isadore "Izzy" Cohen, Percival "Pinky" Pinkerton, and Gabriel Jones).
Debut: Captain America/Steve Rogers, Red Skull/Johann Schmidt, and Captain Samuel "Happy Sam" Sawyer.
Cameo: Sgt. Fury and his Howling Commandos (Nick Fury, Timothy "Dum Dum" Dugan, Robert "Rebel" Ralston, Isadore "Izzy" Cohen, Percival "Pinky" Pinkerton, and Gabriel Jones).
When the Red Skull activates his robot, The Sleeper, he exclaims "The Sleeper has awakened". This is the same line that Kyle McLachlan says in the movie Dune (1984), "Father, the Sleeper has awakened".
Red Skulls robot which is called the sleeper had a skull on it which looks similar to the punisher's skull on his outfit.