When Jess and her Dad go into town to get sunflower seeds for planting, they pull in to the feed store and there is mud "sprayed" evenly on the sides of the vehicle, toward the bottom third. Mud usually patterns around the wheel wells and totally up the back side of the vehicle in this rural environment, not evenly distributed. When they arrive back at the house the vehicle has no mud splatters on it.
After Jess encounters the girl in the basement and runs outside, she comes back inside and slides down the door. The doorknob is clearly missing from the door.
When Ben points out the window, as his mom questions him, the curtain changes from closed/open/closed.
The first time Jess goes to the feed store, the first time we see the bulletin board there is no blood drive notice on it, after she goes to look at it, the blood drive notice has appeared.
When Jess is attacked in the basement, she's wearing work boots and when she runs out on the porch and goes back inside and slides down to the floor she's wearing sneakers.
In the film they are harvesting sunflowers when they are full bloom. Sunflowers can only be harvested in the winter when they are fully black
Sunflowers are harvested after they have gone dry and hang heads down, like mentioned. However, they are harvested with regular combines that are used to harvest wheat, rye, or corn, and not a windrower that is used for putting up hay.
It takes at least 4 months to plant, cultivate and harvest sunflowers, yet Ben never ages throughout the movie.
When Roy dashes to the hall to run upstairs, he braces himself on the wall before beginning his climb. The wall obviously moves when he hits it.
John sees the real-estate agent standing above him in a flashback. It was mentioned that John's family went missing several years ago, but in the beginning scenes of the movie, the real-estate agent hasn't aged at all. He looks the exact same.
Impossivle fingernails marks on the floor. - Kristen Stewart has extremely short nails, in one scene she is dragged into the basement leaving nail marks on the hardwood floor which would be impossible with the fingernails she has throughout the film.
Impossivle fingernails marks on the floor.
In the scene where Roy is removing the sunflower seed bags from the back of the Jeep, you can see the crows on the hatchback of the vehicle with chords tied to their legs.
The basement door changes locations several times within the film, most significantly appearing at the end of a hallway during a haunting scene and along a hallway wall in all others.
The ambulance that takes Jess to the hospital has "Balcarres EMS Prairie Ambulance" on the side of it. Balcarres is in Saskatchewan, Canada, the filming location, about 150 miles away from North Dakota, where the story is set.
When Roy informs Jess that the doctor said her wounds looked self-inflicted, he refers to the doctor as a "he". The doctor which said that was a woman.