- The New Line Platinum Series DVD contains several deleted scenes, including: An extended opening scene establishing Prague. A flashback sequence showing Blade's first encounter with Whistler, part of which can be seen in the film's title credits. An extended version of the ninja fight in the warehouse, with Blade using some fencing tactics to keep Asad at bay. A scene of Whistler shying away from the growing daylight outside. An alternate take of Blade's first meeting with Damaskinos, with Damaskinos wearing, according to Guillermo del Toro, a "Michael Bolton wig". A scene in which Damaskinos explains the effects vampirism has had on him. A much longer version of the House of Pain sequence, including a scene with Nyssa finding a room upstairs with a man unpacking human entrails from a box, a scene that was meant for Michael Jackson. A line by Whistler about "the power of the pussy" A Bloodpack "meeting" of sorts, with Chupa attempting to urge the others to kill Blade and go after the Reapers on their own. A scene in a bathroom after the House of Pain sequence of Lighthammer discovering how far his infection with the Reaper strain has gone. An extended scene of Damaskinos' dinner and blood bath, in which he tells of the fate of his human heart. An extended scene of the lawyer's torture of Blade A "dirty" version of the final scene, in which semen is streaked on the windows that was digitally removed in the final cut of the film because test audiences complained.
- As of 2013, the SyFy Channel cable TV version of the film did the usual time and content editing and left the ending where Rush is attacked and killed by Blade in the adult entertainment theater venue. As of 2016 and 2017, often re-airings of the film end after Nyssa dies in Blade's arms after he mercy kills her by putting her out in the sun.
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