The white markings on Little Blackie's legs and face change throughout the movie.
After Rooster and Le Boeuf won't let her on the ferry and Mattie rides her horse across the river, her clothes are perfectly dry.
At dinner with Mattie & Chen, Rooster angrily tosses his finished plate atop the stew pot and a chunk of bread tumbles off onto the table. Different size chunks of bread reappear and disappear in subsequent shots.
When LaBoeuf and Rooster are discussing the venture at Chen Lee's, Mattie repeatedly changes position between shots.
During the hanging scene, the song "Amazing Grace" is being sung. The scene switches to some kids on swings, and the line "How sweet the sound" is sung in the middle of another verse, then the song goes back to the verse that was being sung.
When Mattie tries unsuccessfully to shoot Tom Chaney, she later says her revolver failed because Rooster Cogburn reloaded it in a state of drunkenness. This only happened in the book when Rooster shot the rat twice with Mattie's gun and tried to reload it, but in the movie, Rooster shoots the rat with his own Colt Single Action Army.
When John Wayne tells that he shot Ned Pepper in the lower lip ,the close up scene of Ned Pepper shows it was his upper lip that looked shot not his lower lip.
When LeBeouf shows up at the hideout after he and Rooster ride out over the hill, his Sharps Buffalo rifle has duct tape around the stock. Duct tape is a twentieth-century innovation.
At the cabin, while waiting for Ned Pepper, Rooster tells Mattie he lost his eye in the battle of Lone Jack, Missouri, just outside of Kansas City. Kansas City was not given its present name until 1889. Whilst this is technically true for Kansas City, Kansas (although part of the current city had been known unofficially as Kansas City in the past), Kansas City, Missouri was incorporated in 1853.
When Rooster hits La Boeuf with his rifle right after Mattie is captured, the rifle bends. It is obviously a rubber gun.
In the shootout between Rooster and Pepper's gang, the film inverts for a few seconds. Rooster's eye patch moves to the right eye, the bandana switches sides, and his rifle and pistol change hands.
When Mattie is riding her horse across the river, the water doesn't appear to be flowing, indicating this sequence was shot in a lake or pond.
When Mattie's revolver is pointed toward the camera, one can clearly see the chambers are empty and cannot be fired.
Campbell carried a Sharps Carbine with a short barrel, whereas the Sharps buffalo rifle has a long barrel.
When Chaney is waiting for the ranger and Mattie to return from watching Rooster and Ned's gang shoot it out, he picks up a rock and uses it on the ranger. One wonders why he didn't just recover the rifle that had been tossed aside earlier.
When Mattie throws hot water on Chaney and runs, he gives chase. Seen from behind, she's under or almost under the roof of the little corral as he's coming up. Then, when they switch shots to showing it from the front, they are farther back.
Glen Campbell's character, "La Boeuf," carries a Hollywood-esque French surname. "Boeuf" is a French world roughly translated as "beef," more accurately translated as a castrated steer. It's undoubtedly male. French nouns are either masculine or feminine in gender, and the articles and adjectives appended to such nouns must match the gender of the noun. "La Boeuf" is not true French; should this name ever show up in the French language, it would be written as "Le Boeuf."
When La Boeuf shoots at the turkey, there is no detectable recoil from his "big bore" Sharps carbine.
When Rooster's horse is shot while underneath him, the stuntman that falls with the horse uses the reigns to intentionally keep the horse from getting back up, making it appear that he's trapped.
Rooster reports Lucky Ned Pepper had robbed the KATY Flyer, a train that did not start running until 1896, long after the time in which the story is set.
When Mattie is emerging from her sleeping area when they are close to Lucky Ned Pepper's hideout, it is evident that she is wearing a form of black nylon tights. Not something you would see in the 1870s.
When Mattie receives the money from Stonehill, the money is clearly 20th century issued US currency, and it is both smaller in size and much less intricate than the currency issued by the federal government in the years following the US Civil War.
Although the date on Frank Ross's grave indicates he died in 1880, the rifle Cogburn carries is a Winchester Model 1892 saddle carbine.
When Mattie visits Little Blackie at the corral prior to the purchase, electrical power lines can be clearly seen in the background.
After the characters in Fort Smith, Arkansas ride into Indian territory, there are many shots of rocky peaks and snow-covered mountains. There are no such mountains within several hours' automobile drive of Fort Smith. Fort Smith is located in the Arkansas River Valley with the Ozark Mountains to the north and the Ouachita Mountains to the south. Both the Ozarks and the Ouachitas extend into eastern Oklahoma. The Boston Mountains north of town, which are part of the Ozarks, are green, tree-covered mountains. The Winding Stair Mountains in Oklahoma, where much of the action takes place, are in the Ouachitas. They are about 70 highway miles from Fort Smith.
Several of the types of pine trees shown in the film only exist in the American West. This is especially noticeable when White Bark Pines are shown. This species are only known to exist in the Western US and Northern Mexico.
The mountains where the movie was filmed were too high to be the Oklahoma and Arkansas areas where the narrative is set. The mountains in those areas top out in the 2-3 thousand foot range. The lowest mountains in the film often visibly exceed that height range.
The movie shows Rooster, Matty and a Beouf riding through stands of Quaking Aspen and Doug Firs, neither of which exist in Oklahoma.
When Rooster rescues Mattie from the snake-infested hole, she insists that he grab her father's gun. But that gun was left behind along the creek where she shot Tom Cheney and was captured.
(at around 1 min) The boom mic's shadow is clearly seen on La Boeuf as he sits down by the dinner table after taking off his spurs.
Mattie Ross' gun is a Colt Walker, not a Colt Dragoon as stated by Rooster. (The movie's producers wanted to use the larger Walker to enhance the image of a little girl using such a large gun.)
Rooster mentions that he lived for some years in Cairo, Illinois, but he mispronounces the name of the town. The local pronunciation is KAY-row.
Chaney claims to have broken ribs after being shot, but later the same day walks, runs, and even tackles Mattie to the ground with no apparent pain.
As Moon lies dying on the floor of the hideout, he talks about his brother, George Garrett, "A Methodist circus rider out of Austin, Texas." The correct term is "circuit rider."
When Quincy is killed in the cabin he is lying on the ground and still breathing.