Goofs

When the adventurers are first imprisoned in the hut, they have no luggage; the room is bare. But when the guide goes back supposedly for the gold necklace, suddenly their luggage is there, including a case of dynamite.

When Old Malla and her escort enter into the prison tent, in one shot it shows Malla entering first. In the next shot, the escort walks in first.

When the adventurers are imprisoned, the outside of the hut is quite different from the inside.

The stock footage used to portray the tribal celebrations shows several different African tribes in various different tribal dresses as well as various different kinds of celebrations.

At the start of the safari, a large-eared African elephant with huge tusks is shown trumpeting from behind. When the travelers look back, shown through the trees is a small-eared Asian elephant with juvenile tusks.

During the ceremony of making the women young again, the process of tapping the pineal gland for hormones involves stabbing the sacrifice at the back of the neck with a short sharp instrument. The pineal gland is actually located deep within the brain.

One of the jungle bird sounds heard in the "African" jungle is a kookaburra, found only in Australia.

While pursuing June, the leopard roars and growls repeatedly. Cats do not growl while stalking or on the chase.

The elephants shown are Asian, not African (based on skull shape).

When the detective shoots his gun into the door handle/lock at the Talbot's house, no bullet hole or damage appears in the door after the shot.

At the camp in the jungle, when the men call loudly, their voices create an echo. This reveals that they are not really in the jungle, but inside a movie studio.

When the dynamite explodes, the tribesmen fall towards the explosion.

The pineal serum (liquid) is so scarce / rare, difficult to obtain (valuable), yet the ring / neck puncturer native who procures it, allows it to immediately dribble down, wastefully from it - presumably exacerbated more when he holds his hand at gravity pulling right angles thereafter the extraction, too.

Many intermixed night/day shots like in an Ed Wood, Jr. film.

When June visits Neil in his office after returning from Africa, Neil asks her why she didn't call him. She replies "I didn't want to bother you." However her lips clearly are saying, "Because I didn't want to bother you."