The title is a parody of the classic announcement made by the train conductor just before the train starts to pull out of the station: All aboard!!!
Granny looks younger and more fashionable here. Her voice characterization is different from later shorts, too.
Tweety is shown before the short begins, by name.
The name of the fictional railroad is the South Eastern and West, which is set to deliver Tweety to Pasadena, California.
Sylvester's fourth wall "Mother!" would also be used by his castmate, Daffy.