- He made his stage debut in 1877 and it followed many engagements at different theaters in the next years, among them the Thalia Theater. He became favored by the audience in no time with comedic plays in which he predominantly took part.
- In the 20s he concentrated again to the stage exclusively and he had his own troupe.
- The actor Guido Thielscher became very popular as a comedian on the stage.
- When he was engaged at the Deutsches Theater in Berlin he could break out of the comedy business and he appeared in several classic plays.
- In 1934 he retired from from the stage.
- To fulfill his his ambition to become a performing artist he took acting lessons by Heinrich Oberländer and singing lessons by Ferdinand Gumbert.
- Guido Thielscher remained busy at the theater till the old age and he continued to entertain the audience.
- The comedy remained his real passion and he continued to act on numerous stages as a comedian. In 1915 they tried to use his popularity in the film business as well and he became his own movie serial.
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