- Had been a court physician under of Tsar Alexander II and Czar Aleksandr III.
- Nephew of Vasily Botkin (1812 - 1869) and Mikhail Botkin (1839 - 1914).
- Father of Gleb Botkin (1900-1969), Tatiana Botkina (1899-1985), Dimitri Botkin and Yuri Botkin.
- Son of Sergey Botkin (1832-1889) and Anastasia Kryloff (b.1839).
- Brother of Peter Botkin (1865 - 1933) and Alexandre Sergeyevich Botkin (1866-1936).
- Father-in-law of Nadine Konchin Botkin (1895-1968).
- Grandfather of Marina Botkina Schweitzer, Nikita Botkin (1926 - 2001), Yevgeny Botkin, Peter BotkinEugene Botkin (1921-1977), Constantin Melnik (1927-2014) and Constantine Botkina.
- Great-grandfather of Alexander George Botkin (1949-2003), Clinton Botkin, Constantine Schweitzer, Sandra Holcomb, Kris Cox and Theresa Kendall.
- Dr. Botkin was portrayed in Rasputin dark servant of destiny by David Warner.
- In the early 1990s, after the unmarked gravesite had been discovered and Botkin's remains were examined, he was found to have had bullet wounds on his legs, pelvis, vertebrae, and forehead.
- The letter was interrupted when Commander Yakov Yurovsky, the head of the command at the Ipatiev House, knocked on Botkin's door. He ordered the entire Romanov party to dress and come downstairs, on the premise that there was gunfire in the town, and they were to be evacuated. But the entire family and their servants (including Botkin) were murdered a short time later.
- Dr. Botkin features as a character in D. Logan's play, Ekaterinburg (2013). It explores the time in captivity of the Romanovs and their retainers in the Ipatiev House in Ekaterinburg.
- Botkin felt it was his duty to accompany the Romanovs into exile, not only because of his responsibility to his patients, the Romanov family, but also to his country. Botkin was considered a friend by Tsar Nicholas II. The doctor also often spoke with Tsarina Alexandra in her native German and acted as a translator for her when she received a Russian delegation.
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