After Anne spills milk over the coat, she starts to clean the chair and puts the glass back on the table. It vanishes in the next shot.
When the thief comes the first time, Margot Frank has a cough. She coughs into a handkerchief. In the next shot of her, her father Otto Frank tosses her a handkerchief because she is coughing into her hand.
On the first night of Hanukkah, Anne says it is December 7, 1942 - a Monday - and two candles are lit and blown out. When Otto Frank goes to inspect the break-in, he says it is Saturday and no one will return until Monday. The same two extinguished candles are seen throughout the scene.
Anne Frank was given the diary for her thirteenth birthday, a few days before they all went into hiding and not after going into hiding as depicted in the film.
Many incidents from the diary are depicted out of order, and some specific speeches and actions are attributed to the wrong persons.
Dr. Dussel mentions in conversation that he, along with his father and grandfather, were born in Holland. All of them were actually born in Germany.
Anne says at one point that it's Wednesday, July 2, 1944, when in fact, July 2, 1944, was a Sunday.
It is known that Margot Frank wore eyeglasses. Diane Baker isn't wearing eyeglasses.
When Anne and Otto Frank begin to hum and dance, the closed captioning says "The Blue Danube", which is wrong. The song they hum to is "Tales from The Vienna Woods". These two songs sound similar because they were both written by Johann Strauss.