(at around 9 mins) When Anita is pulled from the pond, the book she was carrying has become a handbag.
(at around 9 mins) In the park, Roger is dragged away from the park bench where his hat is sitting on it at his back far away from the bench, but is shown wearing it seconds later in the SAME panned/tilt-up shot - without pick it up from the bench.
The news headline on the back of the paper Cruella is holding in bed changes mid-scene. It goes from "Gunboys In Hyde Park Hold Up" to "Page One Baby - All Well"
When the dogs are moving up the frozen creek, Lucky's ears change from white to black.
(at around 26 mins) After watching TV, when the puppies are going off to bed, when Pongo counts puppies 8 through 14 (seven puppies), there are only five puppies, including Rolly, who actually go through the door.
When Cruella takes the wine bottle from Jasper, she throws it into the fireplace causing it to explode with a loud bang. Wine doesn't have enough alcohol in it to explode even if aerosolized like this.
When trying to gain entry to steal the puppies, Jasper and Horace say they are from the gas company. In the sixties all energy production and supply in the UK was in the hands of government-owned regional boards. They would have said that they were from the Gas Board.
When Sgt. Tibs first enters Hell Hall, the main hall is shown. The stairway is to the left, it goes up to a landing, then turns right. Later the Baduns chase Tibs and the puppies, it appears the hall has been mirrored with the stairs being on the opposite side of the hallway as they turn left. But just as the camera pans down and to the right before zooming in on the double doors and the hole in the wall, the stairway the puppies run up as they are running from Jasper, and Horace is briefly shown on the right corner of the shot.
(at around 34 mins) When Pongo starts howling to send out the last part of the Twilight Bark, his four paws temporarily do not make contact with the ground; giving the image of walking on thin air before being pulled back to the ground by his owner.
In the title screen, you can see that all of the pictures are different poses of Pongo. But looking closer, some are clearly designed to be Perdita, especially when in the same pose together, when she was designed/spotted to be Pongo; despite the slimmer body.
At around 29 minutes: during the raid on the house, Nanny lands up in the attic with a picture frame round her neck. She takes it off and puts it down to her left, whereupon it vanishes.
Kanine Krunchies sponsors the Thunderbolt show but in the 1960s, advertisers could not directly sponsor programs in the UK.
In the beginning before the title appears, you see Pongo barking with a hat in his mouth. There is no way he could have done this without the hat falling out.
(at around 12 mins) After Perdita goes into hiding when Cruella first arrives, the camera cuts to Roger while he's saying "Cruella De Vil", but while he's saying it, his mouth doesn't move.
The puppies have American accents, even though the film is set in England and their parents and owners (and indeed virtually every other character) have English accents.
(at around 1h 12 mins) When the truck is being repaired, the sign at the garage says "AAA" (American Auto Association), instead of the British "AA" (Automobile Association).
(at around 36 mins) During the Twilight Bark, there is a scene showing St Paul's Cathedral across the other side of the River Thames. This means that the view must be facing north. However there is a full Moon near the horizon in the background, which would not be seen in a north-facing view.
While girder bridges of the type Cruella fails to navigate during the final chase are commonplace in the Americas, no such bridges are found in East Anglia.
Pongo and Perdita set off from London to Suffolk (north), but when dawn breaks we see them running left to right across the screen, with the dawn breaking in the background. Since dawn is in the southeast in England in the winter, this means they are running roughly southwest.
Following the puppies' abduction, Roger naturally suspects Cruella. Anita tells him she (Cruella) has already been investigated by Scotland Yard, when only moments before, in the same scene, she receives a phone call from Cruella, who says she found out about the kidnapping in the paper and to inquire about whether or not the Darlings had called the police. This should have aroused Anita's suspicions.
Roger writes a hit song that drags Cruella through the mud, a song which refers to her by name, which could lead to her suing him for slander and even possibly legally claiming all of the dogs. In such a case, the law would side with her despite how disliked she is by the public and her peers.
There are base lines (the ones used to draw faces and then deleted) visible in some puppies. The most visible and easy to watch are when they escape walking through the frozen water.