The Green Lantern in this movie is Jessica Cruz instead of John Stewart who was Green Lantern on Justice League (2001) and Justice League Unlimited (2004). This version of Jessica Cruz will have a form of depression that makes her afraid of most things outside of her house, but is able to temporarily shake it off to wield her power ring.
The news report of Superman's battle with Mano, Tharok, and Persuader states that the location is the Maggin Center. No doubt this is a reference to longtime Superman comic book writer Elliot S. Maggin.
This iteration of Star Boy has the power to manipulate mass, making things heavier or lighter, instead of his canonical Legion of Super Heroes power to make things heavier. Also his given name is Thomas Kallor rather than simply Thom Kallor, as it is in the comics.
Several ways the Fatal Five are depicted significantly different from the source material: Mano is the leader rather than Tharok. The Emerald Eye is shown effecting Mon-El. In the comics, its power is based on Kryptonite and Mon-El is one of the few Legionnaires immune to it. The Emerald Eye's power is being related to Green Lantern technology. Validus is shown as slightly taller than human with equivalent strength to Superman rather than being around 50 ft. tall and much more powerful. Tharok can create weaponry from his body. He does not have this ability in the comics. Mano and the Empress are in a relationship.