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| Episode cast overview, first billed only: | |||
| Zachary Levi | ... | Chuck Bartowski | |
| Yvonne Strahovski | ... | Sarah Walker | |
| Joshua Gomez | ... | Morgan Grimes | |
| Sarah Lancaster | ... | Ellie Bartowski | |
| Adam Baldwin | ... | John Casey | |
| Rachel Bilson | ... | Lou | |
| Kevin Weisman | ... | Reardon Paine | |
| C.S. Lee | ... | Harry Tang | |
| Bonita Friedericy | ... | General Diane Beckman | |
| Emy Coligado | ... | Poppy Tang | |
| Ryan McPartlin | ... | Devon 'Captain Awesome' Woodcomb | |
| Mark Christopher Lawrence | ... | Big Mike | |
| Vik Sahay | ... | Lester Patel | |
| Scott Krinsky | ... | Jeff Barnes | |
| Sanjay Chandani | ... | Mason Whitney (as Sanjaya Chandani) | |
A spy after nuclear secrets poisons a scientist to obtain the information. But when scientist collapses on the street, he discreetly passes the chip on to Ellie. Then the spy goes after Ellie poisoning her. As team Chuck sets a trap for him, they all get exposed to the poison. Now they all need the cure. The poison compels them to tell the truth, which creates a dangerous situation for Chuck and Sarah. Written by Jesse Sanchez