There are special scene transitions for this episode, cards and poker chips spread across the screen to go from the Vegas scenes to the scenes at the Jeffersonian. The crazy socks that Booth has on in this episode was a homage to David Boreanaz. In real life, he loves to wear crazy and bold socks, even with suits. David Boreanaz was also an underground fighter in the Angel Season 1 episode "The Ring". In that show, he was made to fight against his will and was almost defeated in his main fight against a much larger foe.
T.J. Thynes character Jack Hodgins mentions that a chemical, hexavalent chromium, was found in his bugs. Just 6 years earlier he played a lawyer in "Erin Brockovich" which involved a town contaminated with hexavalent chromium.
This episode marks the first use of Dr. Brennan's blood-revealing flashlight. In reality, the chemical luminol is applied to crime scenes to reveal traces of blood. UV lights, like Brennan's, don't reveal blood, but they do reveal some other bodily fluids (semen, urine, and saliva). UV light also makes bone fragments glow, so it is likely that she would carry the flashlight.
Booth wears a Guys and Dolls shirt - the musical is about a set of gamblers with an underground craps game. In this episode, Booth and Brennan are in search of an underground fight club. It is also revealed in this episode that Booth's game, back in his gambling days, was craps.