Otis and the animals put on a fake commercial to trick the Farmer into going to the made-up "TurnipCon," allowing Otis to throw himself a giant birthday bash, but the plan backfires when Snotty Boy is hired to "barn-sit."
The animals accidentally get Mrs. Beady committed to a mental institution, which makes Otis happy until he hears what happens to people in those places. He vows to spring her, but when they're seen, she starts going crazy!
Otis has a blast hypnotizing his barnyard pals into doing silly, demeaning things. The animals' attempts to get him back backfire when Otis is hypnotized into destroying the farmer whenever he hears a bell.
When the barn's 50-year-old safety film melts in the projector, Otis vows to make a new one and become a famous movie director in the process. His blind ambition causes his barnyard cast to get injured so often that they quit!
Mrs. Beady videotapes the animals walking and talking and is going to air her footage on live TV! Otis and the crew pirate the TV signal and broadcast their own show to bide time while they try to steal back the evidence.
It's Moose Appreciation Week, so Otis disguises himself as a moose to get in on the freebies and handouts. Unfortunately, by the time he gets his costume together, it's the next day -- the official start of Moose Hunting Season.
Otis and the gang become overnight pop sensations when Pip broadcasts a performance of a song they wrote and performed at the Saloon on the spur of the moment. Peck quits in disgust over the band members' egos.
After Snotty Boy is knocked unconscious while terrorizing the barnyard, Otis sends a disguised Pig over to the Beadys' as Snotty Boy so they don't get suspicious. But Pig prefers living there and refuses to come home.
After Otis accidentally destroys Pip's mouse hole, Pip tries moving in with other members of the barnyard, all ending badly. Concluding that the other animals have grown sick of him, he runs away to live in a bee hive.
Otis gets a letter from his childhood bully saying he's "coming" for him. Otis freaks remembering all the beat-downs at his bully's hands. With the help of his friends, Otis decides to confront the bully.
Peck takes a fake potion to get enough courage to speak to his crush, Hannah, but he ends up being so confident that he challenges Root, a rooster much bigger than him, to a duel./ Bigfoot is sighted in the woods, and Otis decides to go hunting for him, but Abby finds him and brings him to the barnyard for his protection. Mrs. Beady, however, is out to capture Bigfoot and prove she is not crazy.
When Cowman is assigned the task of guarding the Jurassic Corn Kernel at the county fair, Professor Mertin Fargleman (Tom Kenny) wants to steal it to win the Gardening Award posing as Professor Twineyvines. When the town is impressed with Twineyvines and unimpressed with Cowman trying to save the Kernel from him, Twineyvines becomes the new protector. Immediately, Cowman takes the kernel to protect it and is branded a thief and then chased by a mob. He then decides to stop Twineyvines when Cowgirl finds Cowman and Ratboy headed for Peru and says that Green Rooster, Mr...
Otis is forced to take over teaching the kid animals after he injures their teacher in a scooter stunt gone wrong. Not big on book-learnin', Otis tosses away the lesson plan and decides to teach the kids how to have fun.
When Pig starts acting crazily to his barnyard friends, he explains that the behavior is caused by his hormonal need to return to his spawning grounds and marry. The animals accompany him to his birthplace and meet his bride.
Otis tells Abby that girls get all the breaks, and then sets out to prove it by dressing as a girl to go bowling with his pals. But they run into Snotty Boy and his friends, who pressure them into going on a pizza date.