Many of the scenes from the trailer were not in the movie, including Brad Pitt riding a bike while shooting and Keith David ordering someone to "bring in everyone," after finding out "it's the Smiths."
The shot of the helicopter flying over Bogotá at the start of the flashback sequence was originally used in Clear and Present Danger (1994). For its appearance in Mr. & Mrs. Smith (2005), CGI was used to enhance it, with burning buildings and someone sitting in the rear passenger seat of the chopper.
Michelle Monaghan (Gwen) went on to star opposite Tom Cruise in Mission: Impossible III (2006). At one point, it was rumored that Brad Pitt would replace Cruise in the "Mission: Impossible" film franchise. However, the rumor was false, as Cruise continued to star throughout the franchise.
The film has been considered to be a remake/updated version of True Lies (1994). In that film, Helen Tasker (Jamie Lee Curtis) a bored housewife is not aware that her husband Harry (Arnold Schwarzenegger) is a secret agent.
Kost Mart, the name of the department store John and Jane are hiding in towards the end of the film and it is the same name of the wholesale retail store where Dick Harper briefly worked at in the movie Fun with Dick and Jane.
The coffee shop scene where Brad Pitt and Angelina Jolie confront Vince Vaughn was filmed in the same coffee shop as a scene in Old School, also starring Vince Vaughn and Perrey Reeves. A coffee shop scene in Gone in 60 Seconds where Nicolas Cage talks with his mother, Grace Zabriskie, also used the same location, and also stars Angelina Jolie.
Eddie turns down a bounty of $400,000 because he won't get out of bed for anything less than $500,000 is borrowed from an infamous quote by Linda Evangelista in a 1990 interview by Vogue and how she won't "wake up for less than $10,000 a day"
The trivia items below may give away important plot points.
After the Smiths discover each other's identities, Jane speeds away in the car and John runs after her. He ends up tripping and crashing through a fence, and his gun goes off. The trip was a mistake, and Brad Pitt's reaction, as well as his following ad-lib, "Oh dear God," were genuine.
The original ending featured villains played by Jacqueline Bisset and Terence Stamp. After this was dropped, a second ending was filmed with Angela Bassett and Keith David as the villains. This, too, was dropped when it was decided that the film did not need a final confrontation with the villains at all.