(at around 7 mins) The "And twins!" sequence is a parody of a 2003 ad campaign by Coors Light beer. Diane Klimaszewski and Elaine Klimaszewski reprise their roles as the Coors Light Twins from the ads.
Jason Friedberg and Aaron Seltzer were briefly in talks to write and direct the movie after the Wayans Brothers left the franchise. However, Friedberg and Seltzer wanted to make the movie chiefly a parody of Star Wars, Harry Potter and The Lord of the Rings, whose working title was reported as "Scary Movie 3: Episode I: Lord of the Brooms." Ultimately, Harvey Weinstein preferred to keep the series parodying horror films, and so Friedberg and Seltzer's script went unused.
(at around 1 min) In the beginning of the movie, the scene where Pamela Anderson and Jenny McCarthy are discussing what happens on the tape, McCarthy's character mentions something going on on a boat and a car. She is referring to Anderson's and Tommy Lee's famous "private" video that got leaked online.
Some additional scenes, extensively including spoofs of The Matrix Reloaded (2003), were removed from the final film. They included a scene where Cindy fights a large number of Tabithas, similar to the Burly Brawl scene in The Matrix Reloaded (2003) and Orpheus "prophesizing." The removal of those scenes is also the reason of Queen Latifah never appearing in the final movie dressed as Neo from The Matrix Reloaded (2003), although she appears dressed up that way in the movie's promotions and poster.
(at around 57 mins) When Leslie Nielsen wondered what would President Ford would do if faced with a difficult situation, he is not staring at a portrait of President Gerald Ford but Harrison Ford. Ford previously played a US President in the film Air Force One (1997) which at times utilized an alternate poster similar to Ford's portrait in this film. Moreover, Nielsen previously appeared in the parody film Wrongfully Accused (1998) which spoofed The Fugitive (1993), a film starring Harrison Ford.
(at around 53 mins) When Cindy is searching about Morton Lighthouse, there are several pop-ups that appear on the screen, but one is not an ad. It says "This may sound overly simplified... but high interest rates eventually cause low interest rates... and conversely... low interest rates eventually lead to high interest rates," with a yellow background.
(at around 1h 13 mins) The scene where president (Leslie Nielsen) opens the door and says "I wanted to say, good luck, we're all counting on you" is referring to the cockpit scene of the movie Airplane! (1980) where Leslie Nielsen says the same thing.
(at around 43 mins) When Orpheus is trying to watch the Lakers game, ShaNeequa tells him the Lakers will win by twelve. This mostly likely refers to the February 1, 2003 game against the Utah Jazz, which resulted in a 99-87 win for the Lakers at home.