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In Gotham, one year after the defeat of Two-Face and the Riddler, Batman and Robin attempt to thwart Mr. Freeze from robbing diamonds from the natural history museum, but he steals one and flees. Mr. Freeze was originally Dr. Victor Fries, a doctor working to develop a cure for MacGregor's syndrome to heal his terminally ill wife. Fries was forced to wear a cryogenic suit powered by diamonds after becoming unable to live at normal temperatures following a lab accident.
At a Wayne Enterprises lab in Brazil, the botanist Dr. Pamela Isley is working under the deranged Dr. Jason Woodrue, experimenting with the Venom drug. She witnesses Woodrue use the formula to turn the violent, but diminutive, convicted serial murderer Antonio Diego into a hulking monstrosity dubbed "Bane". Woodrue and Isley argue over the use of the drug which ends when Woodrue attempts to kill her by overturning a shelf of various toxins. Despite Woodrue's efforts, she is resurrected, transforming into the beautiful ...