On the DVD "Scene Selection" menu, Bill Moseley's character is standing in the grave yard speaking about facts of life. During his speech, he will start to tap his feet and you will hear echos as if he is standing on concrete floor and not in a dirt cemetery as his character is setup to be.
When the side window of the car driven by the four victims is broken, it's regular pane glass, yet cars of that age use tempered glass. Later scenes of the same car with broken windows also show broken tempered glass.
When Captain Spaulding and Stucky are having their conversation in the very beginning and Stucky is handed the bathroom key it is on a hand that is flipping the bird, however when the gunmen pull him out of the bathroom he is holding the key on a hand that is giving the devil horns and also missing his glasses.
When Mother Firefly pulls the pistol on Lt. Wydell the revolver seems very large, and the hammer has been cocked back. When she fires, the hammer is already down and does not drop. The gun also appears to be a different, smaller weapon when the scene cuts to Wydell being struck and killed.
When Bill is shown getting his right arm cut off, the ax hits the obviously fake arm and turns sideways. It does not go all the way through, but the ax wielder is shown immediately afterwards holding the neatly severed limb after only the initial swing.