The location of Townsville is given in the episode The Powerpuff Girls: Him Diddle Riddle (2001) when the girls must solve numerous puzzles given by Him, "or else the Professor will pay." The final location in the hunt is given by the Mayor who states that the global coordinates of an intersection is at 32 degrees north and 212 degrees west (or 148 east). This puts Townsville in the Pacific Ocean, approximately 400 hundred miles east-by-southeast from Japan's largest island of Honshu.
The trio was originally called Whoopass Stew! (1992), but their name was changed to The Powerpuff Girls (1998) when adult audiences disapproved of children hearing this profanity to younger generations, especially pre-school, kindergarten and first grade, so they would not speak profanity in school, without realizing they are or were cursing.
In "Nuthin' Special" cartoon of The Powerpuff Girls: Nuthin' Special/Neighbor Hood (2003), Buttercup discovers that she's the only one in Townsville who can roll her tongue. The rest of Townsville try this, going from on person to another. One of these people is Gene Simmons, the bassist/vocalist from the band KISS.
In the first episode, "Three Girls and a Monster," of The Powerpuff Girls: Three Girls and a Monster/Monkey See, Doggie Two (2000) Blossom orders the commencement of flight plan Alpha-Omega-Atari. When executing this plan, the girls race through the city and at one point form the Atari symbol with their color trails. In addition, the names Alpha and Omega are the names of the first and last gun turrets in Atari's classic arcade game Missile Command (Delta is the one left out).
After The Powerpuff Girls Movie (2002), the show underwent a series of changes: the production switched from traditional inked and painted cels to digital post production; the design for Mojo Jojo was changed to the more sinister-looking one used in the movie; and the end graphic, with the concentric hearts and the girls appearing in an explosion of stars, switched to the slightly more elaborate version used at the end of the movie.
In "Slumbering With The Enemy," the girls are looking though a teen magazine during a slumber party. Amongst the pictures of guys, one sees the creator of the show, Craig McCracken. He is the one wearing a black EBay shirt.
In "Collect Her," while the mayor is performing a role call of the population of Townsville, he calls out "Genndy McCracken," an amalgamation of producer Genndy Tartakovsky and Powerpuff Girls creator Craig McCracken.
In season 3 episode 6b (Monkey See, Doggy Two), Mojo Jojo refers to his mistake in the original plan to turn everyone into dogs (Monkey See, Doggy Do). Here, he plays footage that is identical to that of the episode to which Blossom asks if he has cameras planted around the world. Since he agrees, it can be inferred that the show as a whole is taken from footage Mojo Jojo's cameras.
Despite being a kids cartoon, there have been notable deaths in various episodes. Bunny dies in "Twisted Sister", The Rowdyruff boys die in the episode of the same name (only to be revived by Him in a later episode) and Dick Hardly dies in "Knock It Off".
Originally, two adults and a child dubbed the girls' voices in the Brazilian version. While Iara Riça (Blossom, as "Florzinha") and Christiane Monteiro (Bubbles, as "Lindinha") were adults, Luísa Palomanes was aged 11 when she started voicing Buttercup, as "Docinho."
Jim Cummings and Tom Kenny's second TV voice role after CatDog (1998). They wouldn't work together again until Winnie the Pooh (2011) where Jim Cummings did Pooh and Tigger while Tom Kenny provided the voice of Rabbit.