The light cycle sequence originally had background music. This alternate scene is present on the special laserdisc edition.
Some non-USA versions of the film have a brief prologue of text explaining the difference between the computer world and the real world right before the starting credits.
Trailers feature a deleted scene, where Flinn de-rezzes an unknown program on the ring-game with a direct hit.
The 20th Anniversary DVD edition includes a deleted love scene: Tron and Yori go to her apartment, where she pushes a button on the wall, the walls disappears and her uniform changes into a different costume. Tron ask her " How do we get into the I/O Tower". She replies "First, you have to go all the way down" as she moves her hand down Tron's body. Also included are a partially completed "morning after" scene (after Tron visits Yori's apartment, Tron tells her "It's time to go"; however all dialog tracks for this short scene are lost), and the "light cycles" scene with its music (music that also shows up in the "here goes nothing" scene, and which Wendy Carlos would later use as the basis of one movement of her "Moonscapes" suite), on the auxiliary DVD. Also included on the auxiliary DVD is a new 90-minute "making of" feature, with cast and crew interviews, and an alternate version of the end credits, with Wendy's close music playing in its entirety, instead of cutting to Journey after the organ solo.
Also included in the DVD is the original "Opening Monologue" from the original theatrical release, a la Blade Runner, that "explained" the world that the viewer was about to enter. It said: 'This is the story of two worlds and the beings who inhabit them. One of these is our world, the one we can see and feel. The world of the "Users". It lies on our side of the video screen.' The next card read: 'The other, an electronic micro-civilization, lives and breathes just beyond our grasp. This is the world of the "Programs." Because we, the Users, have created this new world, part of us lives there, too...' The third, final card reads, in large type: 'ON THE OTHER SIDE OF THE SCREEN'. This occurs between the Buena Vista and Walt Disney credits from the original screen. This was put in the international English language release, and the subsequent domestic wide release in the United States.
When the film was released in theaters, there was an alternate prologue which has since been excised. Similar to the prologue on the 20th Anniversary DVD, the alternate prologue states that every time we renew our driver's license, pay our telephone bill, or use a credit card, a bit of each of us is left within the computer. This prologue is not on the home video versions, nor on the 20th Anniversary DVD.