The Mad Hatter has escaped from prison, and is on a quest to add Batman's cowl to his collection of hats. He attends Gotham City's annual headdress ball disguised as the Three-tailed Pasha Of Panchagorum, and snatches a large ruby off of columnist Hattie Hatfield's headdress. The villain makes a getaway, but not before turning Batman's cowl pink with a radioactive spray. He trails Batman to the Atomic Energy Laboratory, knowing he will have a chance to snatch his cowl when it is removed for decontamination.
What appear to be the skeletons of Batman and Robin are found at Gotham City's Atomic Energy Laboratory, where the Mad Hatter left them to irradiate. Word of their demise spreads like wildfire. With the world busy mourning its loss, the Mad Hatter has no trouble snatching a valuable ruby from the Golden Buddha of Bergama's forehead. However, when rumor spreads that Batman and Robin are still alive, the Mad Hatter's scheme appears jeopardized.
The Catwoman manages to drug Robin. The junior member of the Dynamic Duo is now the Catwoman's mindless slave. Batman is on the trail of the sultry villain, but he's hampered by the fact he can't harm his partner. Eventually, Batman is trapped by the Catwoman. He's in a giant trap and Robin is cutting the rope that will cause a giant mouse trap to snap on Batman. The Catwoman offers Batman a choice -- die by Robin's hand or agree to be subjected to the same drug.
Batman agrees to let the Catwoman drug him. But he appears to have a headache and the Catwoman permits him to take an aspirin. After the drug is administered, Batman appears to assist Catwoman on her crime wave. However, the police receive anonymous telephone calls that enable them to pick up members of Catwoman's gang, including the drugged Robin. Meanwhile, the Gotham City police try to trace the hot line to the Batcave. Batman is there with Catwoman. He uses a device that foils the trace. Eventually, Batman reveals he hadn't taken an aspirin but his universal drug ...
As a front for his next crime wave, the Penguin forms a motion picture company. He teams up with Marsha, Queen of Diamonds, and gets Batman and Robin to take part in his movie. Marsha attempts to put Batman under her spell with drugged lipstick during the love scene, and filming at the Gotham City Museum of Antiquities turns out to be a good cover for the Penguin to snatch some ancient armor. Moreover, when the fight scene turns out to be all too real, the Dynamic Duo are captured and tied to a giant catapult about to be sprung.
In order to keep a close eye on the Penguin (and hopefully locate the stolen chain mail armor), Batman and Robin rejoin Penguin's movie efforts. The next part of his film is to take place in medieval times. Therefore, Penguin has the Dynamic Duo dress in armor suits, but only to trap them with a giant magnet while he snatches top-secret papers from the Hexagon. Batman and Robin free themselves and follow the villain, but after a losing battle with him, the Penguin has them hauled off as scrap metal to be crushed.
During a party for Batman's crime-fighting anniversary, the Riddler appears and nabs a golden calf filled with money for charity. His next target is the Gotham City Bank, which he floods for an underwater robbery. Although Batman and Robin try to stop him, the villain makes a clean getaway, leaving only a final riddle that stumps the Caped Crusaders. They discover all too late that his next move is an attempt to destroy them, this time with deadly quicksand disguised as strawberry icing atop a giant cake.
After two more robberies, the Riddler has enough money to purchase Professor Charm's Demolecularizer. He demonstrates his weapon on a park statue, then issues an ultimatum - if all criminal statutes are not rescinded within two hours, he will begin dissolving every building in Gotham City. He rigs his device to automatically fire on police headquarters if his demands are not met. The city's only hope is Batman, who believes he has a plan to counteract the device.
Counterfeit money is being distributed by respectable citizens in Gotham City. Batman and Robin investigate, and find one of the Joker's lifelike robots substituting for a bank teller. They are certain who is behind the crime. But to get actual proof, Batman plans to visit the villain as Bruce Wayne and trick him into disclosing his operation. When his scheme suddenly backfires, it appears he will instead have to appoint the Joker as Vice Chairman of the Gotham National Bank.
The Joker is now Vice Chairman of the Gotham National Bank, due to an unsuccessful plan of Batman's. He installs his robots as tellers, and despite his criminal past, seems to be running things in perfect order. The Dynamic Duo realize they will have to trick him into making a mistake in order to remove him. They go to work on a plan to gain control of his robots. But that plan is jeopardized when, due to an odd turn of events, Batman's true identity Bruce Wayne is declared mentally ill and hauled off in a straight-jacket.
Catwoman is released from prison, and claiming she wants to enrich her education, enrolls at Gotham City University. She dresses one of her own henchmen as the Caped Crusader in order to frame him for a robbery. With Batman in jail, she goes ahead with her plan for a mass sit-in at Chimes Square. Catwoman is more than surprised when Batman himself shows up at the event. However, his efforts to expose the villainess are futile, and she prepares to terminate him and Robin in front of the whole crowd.
Catwoman goes ahead with her scheme to steal several Batagonian Cat's Eye opals, but she has trouble finding someone to fence them since such rare stones would be easily spotted. With little recourse left, she decides to turn them in for the reward money. It seems like the perfect job for her acquaintance, Freddy the Fence. But when he notices that the gems are fakes, Catwoman realizes she has been tricked by Batman and vows to get revenge on the Caped Crusader once and for all.
Colonel Gumm moves out of the Pink Chip Stamps Factory, taking its owner Pinky Pinkston along as a hostage. A message hidden by Miss Pinkston in a bowl of alphabet soup directs Batman and Robin to the International Stamp Exhibition. Though the Green Hornet and Kato will be attending to try to expose Colonel Gumm, Batman and Robin believe they are out for personal profit. The misunderstanding has the two sets of crime fighters fighting each other while the real criminal nearly makes off with the exhibition's rare and valuable stamps.
After a blow to the head, Yale's Egyptology professor once again believes he is King Tut. He mistakes Bruce Wayne's friend Lisa for Queen Cleopatra, and kidnaps her so he can bring her to Egypt as his bride. Batman and Robin soon locate Tut's hideout, thanks to a hidden tracking device. But soon after entering, they are captured by Tut's cronies, and Batman is submerged in a sarcophagus under five feet of water.
Though Batman escapes his watery trap, Robin is left in the hands of King Tut. The deluded villain still believes that Lisa Carson is really Queen Cleopatra, and calls Lisa's father to demand a ransom of $8,300,487.12, the mortgage on the Pyramids. Using the Jolly Jackson radio show to communicate, Tut negotiates the terms of payment with Batman. However, the villain most likely intends to keep both the money and Lisa, and has further plans to fry the Caped Crusaders in his royal boiling oil.
The Black Widow uses her cerebrum short-circuiting device to rob bank after bank in Gotham City. Batman and Robin soon catch up with her, but actually capturing the villainess proves harder than expected. Despite her nerve paralyzer spray, and two remote-control dummies that try to throw the Dynamic Duo off course, they finally make it inside the Black Widow's lair. But soon after entering, they are trapped in a giant web with two deadly black widow spiders.
The Black Widow adjusts her cerebrum short-circuiting device to successfully alter Batman's brain. With Batman powerless to resist, and Robin captured, she disguises herself as the Boy Wonder and brings along a dummy Batman to rob another bank. Commissioner Gordon initially believes Batman is setting a trap for the villainess. But when word comes of the successful robbery, the commissioner puts out an all-points bulletin to bring in the Caped Crusaders dead or alive.
Mr. Freeze is back in town, and has plans to build an icy weapon that will have the world at his control. With help from Glacia Glaze, the renowned ice skater, he captures Professor Isaacson and tries to extract the secret formula for instant ice from him. Mr. Freeze then sends his trained seal Isolde to Police Headquarters with a note, demanding Batman deliver a ransom in exchange for the professor. But the frosty villain not only plans to keep the money and the professor, but to terminate the Dynamic Duo in the process.
Back in his iceberg headquarters, Mr. Freeze persuades the kidnapped Professor Isaacson to build him a thermodynamic ice ray beam. He issues an ultimatum that he will begin freezing the entire country if his demands are not met, then gives a demonstration of his weapon. As the villain works out just what his demands are, Batman and Robin track his seal, Isolde, through an ice-packed Gotham Harbor to locate the villain's hideout.
The Penguin plans to gain immunity from the law by becoming the police commissioner's son-in-law. He kidnaps Commissioner Gordon's daughter, Barbara, and threatens her into agreeing to marry him. Unknown to him, Barbara Gordon has a secret crime fighting identity, Batgirl. Alfred learns of her secret when he is brought to Penguin's hideout instead of a preacher, and Batman and Robin realize just how valuable their new ally is when they find themselves hopelessly strung up in Penguin's lair.
The Riddler is out to control all of prize fighting in Gotham City. He begins by kidnapping and brainwashing top prizefighters with the aid of the Siren. Then, under the guise of Mushi Nebuchadnezzar, supposed boxing champion of Southwest Asia, he challenges Batman to a fight in the ring. But when it becomes clear that the Riddler has no plans to play fair, it's up to Batgirl to help her crime fighting companions.
Lorelei Circe, aka The Siren, launches a two part plan to become the richest and wickedest woman in Gotham City. First she puts Commissioner Gordon under her stunning spell and orders him to stow away in the Batmobile in order to find out the location of the Batcave as well as Batman & Robins secret identities. Secondly she entrances millionaire playboy Bruce Wayne and implores him to sign over all his wealth and belongings to her.
The Penguin teams up with parasol collector Lola Lasagne. Together, they plan to rig the upcoming Bruce Wayne Handicap to make a fortune from Lola's prize racehorse Parasol. They break into Glu Gluten's Glue Factory to steal a condemned horse, planning to switch horses before the race and make their money by betting on the real Parasol. When Batman, Robin, and Batgirl catch up with them at the glue factory, Penguin sneaks outside and plasters the Batmobile with a sticky glue.
The Penguin steals a priceless folio of famous parasols from the Gotham City Library. He plans to wager the $10,000 earned from its ransom on a rigged horse race. Aided by his partner-in-crime Lola Lasagne, he disguises the favored entry Parasol as the unknown "Bumbershoot," then enters a painted glue factory horse as "Parasol". With everyone betting their money on the fake horse, it looks like the Penguin will make a fortune when the real Parasol wins the race. But he wasn't counting on a last-minute entry of Bruce Wayne's, the horse Waynebow, ridden by none other ...
King Tut has been predicting crimes, then secretly making sure they happen in order to gain the police's confidence. He hopes to then send them on a false lead while he commits the crime of the century. After failing an attempt to discover Batman's true identity, he gives his biggest prediction - a jailbreak of several super criminals. While the entire police force is sent to the penitentiary, Tut nabs a collection of priceless Egyptian scrolls from the Gotham City Library. The scrolls contain the key to an ancient statue, rumored to give its possessor control of the ...
Louie the Lilac has cornered the flower market in Gotham City, hoping to gain control over the entire "flower generation." He kidnaps the organizer of their planned flower-in, Princess Primrose, and brainwashes her with his Stupefying Aromatic Spray. Batman and Robin follow a clue to the villain's lair only to be captured by Louie and fed to his giant man-eating lilacs. With the Caped Crusaders out of the way, Louie heads back to the flower-in to continue his evil plan. Alfred, meanwhile, has been alerted to Batman and Robin's situation, and goes looking for Batgirl ...
Egghead and Olga, Queen of the Bessarovian Cossacks, team up to kidnap Commissioner Gordon. In exchange for his safety, they demand a ten cent tax for every egg eaten in Gotham City. Batman and Robin suspect that Olga may also be after the giant Samovar of Genghis Khan and use it to find a way into her hideout. However, it turns out that the Duo have been expected. While Olga targets Batman as her future husband, her cook prepares a wedding borscht which will contain Commissioner Gordon and Robin.
Egghead and Olga, Queen of the Cossacks, steal two pounds of Radium from the Gotham City Radium Center. Using it in their "Zapping Machine," they attempt to hatch an ancient Neosaurus egg, planning to feed the baby dinosaur a meal of Batman, Robin, and Batgirl. Surprisingly, the machine seems to be working. But when the egg cracks, the baby Neosaurus is more than they can handle.
The Joker plans to become the king of surfing, hoping the fame will give him control over the hearts and minds of Gotham City. He captures top surfer Skip Parker, then uses his "Surfing Experience & Ability Transferometer" to transfer the needed skills and stamina from Skip to himself. When all the other contestants drop out of the upcoming surfing match, Batman steps up to challenge the Joker's supremacy.
Commissioner Gordon travels across the channel to Londinium by request of the President to assist in a spree of baffling burglaries. Also aboard ship are Bruce Wayne, Dick Grayson, their manservant Alfred and Gordon's daughter Barbara. Alfred sets up a make-shift Batcave under a rented mansion near Londinium while Batman & Robin visit lord Ffogg and Lady Peasoup, who run a girl's finishing school that teaches their students a degree in shoplifting.
Lord Ffogg and Lady Peasoup plan to steal a ship-worth of mod clothing material with which they can control the fashion world of Londinium. Ffogg and his men subdue Batman, Peasoup and her girls grab Robin and Lady Prudence uses the paralyzing fog on Batgirl.
Batman, ably assisted as always by Alfred, saves Robin from being crushed by a winch at the Tower of Londinium. He also rescues Batgirl from the dungeon at Ffogg Place. But Robin gets stung by an African Death Bee as Lord Ffogg and Lady Peasoup get ready to steal the crown jewels at the Bloody Tower.
The Catwoman targets the fashion industry by first terrorizing a banquet honoring Batgirl and then attacking a fashion show. During the attack on the fashion show, Catwoman captures Batgirl and takes her back to her hideout where she threatens to kill her with a pattern cutter. She then tells Batman that if he attempts to rescue her that it will leave the visiting Queen Bess of Bellgravia vulnerable for attack. Now the Caped Crusader must find a way to save Batgirl and prevent any harm to Queen Bess.
Egghead and Olga, Queen of the Cossacks, kick off their return with several thefts from the Gotham City Museum. Egghead begins planning a series of heists - intended to provide him with the dowry needed to marry Olga. Swiping 500 pounds of dehydrated caviar goes off without a hitch. But when Batgirl attempts to outfox Egghead, she is instead captured by the villain. It's up to Batman and Robin to find the villains' hideout before Olga's Cossacks make mincemeat out of their crime fighting friend.
The Joker, just released from prison, teams up with Catwoman. They follow the clues of an ancient riddle to retrieve a nightshirt and an antique crib, which together form a map to a hidden cache of gunpowder which they plan to use for their next crime. Batman and Robin, together with Batgirl, follow their trail, little knowing that they are about to walk right into an ambush.