Set in 1912, the song "Trouble" mentions both the beverage Bevo (first offered in 1916) and the magazine "Captain Billy's Whiz-Bang" (first published in 1919). "Whiz-Bang" is named for a type of artillery round in World War I (1914-1918)--the publisher was a veteran of that war.
When Harold Hill tells Mrs. Paroo about the "great cornet players ... O'Clarke, O'Mendez, and O'Klein", he's referencing the (obviously non-Irish) real life trumpet/cornet virtuosos Herbert Clarke, Rafael Mendez and Mannie Klein. Mendez and Klein would have been around six and four years old at the time of the story.
In the first town folk song, "Iowa Stubborn", a couple stand with a frame looking exactly like Grant Wood's "American Gothic". Grant would not paint this till 1930, 18 years after the setting of the show.
When Amaryllis is practicing her scales both Marian and her mother hit the correct, lower note to the right of the one Amaryllis had played. On a piano the notes are higher as you move right on the keyboard.
Right before Shipoopi, Mrs. Shinn says her girls are ready to perform, but Ethel dances with Marcellus in the number and is dressed in a flowered dress. Immediately after the number, she is dressed in her Grecian urn outfit, ready to perform.
Chairs in front of the stage move around during the July 4th celebration in the auditorium. First there are three together with one chair a ways off to the left; then later, as one of the school board members prepares to sit down, the four chairs are in a group.
In the candy shop when Hill and Marion are sipping on their strawberry sodas, Hill releases his straw and the straw floats to the top. The camera angle changes with Hill holding the straw and it's at the bottom of the glass.
After waving to the departing salesmen on the (yellow) train he came in on, Harold turns to leave the depot and the only thing behind him is a lonely (brown) freight car sitting still on the track. This same freight car is visible there many days later when the Wells Fargo Wagon comes into town, and four weeks later when anvil salesman Charlie Cowell is leaving town.
Robert Preston's hair goes through several color changes through the course of the movie. At the beginning it's a sandy blond, then it grows darker and by the time of the footbridge scene it's a deep, chocolate brown. Yet minutes later when Prof. Hill is apprehended in front of the Paroo residence it's back to sandy blond.
In the opening sequence, the train is traveling from the Illinois Iowa. The background visual scroll only shows flat cornfields. We never see it crossing a river, let alone the Mississippi, which is the only way to get between the states in question.
When the film opens, a train is show leaving Brighton, IL. The conductor announces this as the last stop in Illinois and the next stop is River City, IA. Brighton is in southern Illinois - farther South than Iowa - on a rail line running to St. Louis, MO.
Marian orders a Strawberry Phosphate in the Soda Shop with Prof Hill, which would have consisted of fruit syrup, acid phosphate drops, and soda water. The drinks they are shown with are not fizzy or carbonated and look much more like fruit juice.
In the opening musical number, "Rock Island", sung by traveling salesmen aboard a moving railroad car, the salesmen are jiggling up and down to the rhythm of the train. But the car and the scenery outside are perfectly steady, revealing that the salesmen are actually dancing, not being joggled by the train.