Before John's hand is chopped off, his wrist is very hairy, but the "stunt wrist" that is chopped has little or no hair (and also doesn't bleed).
Whilst waiting outside the Hollis home, Willis licks and seals the letter for Charlotte. When she opens it, however, it is unsealed.
As household staffers pack up her belongings, a haggard Charlotte wanders through the house wearing no makeup, but the minute she sees an insurance investigator in the yard, she's suddenly wearing lipstick, eye makeup, and looking years younger.
When Miriam is repacking the head in a box whilst talking to Drew, she folds the longer edges of the box first and then the shorter ends. In the next shot she is tying up the sealed box with the longer edges folded over the shorter (the correct way a box will be sealed).
When Miriam meets Jewel in public, a smiling young girl extra with a bouffant hairdo and a ribbon in her hair can be seen walking behind them. Seconds later, the same girl walks behind them again.
Jewel Mayhew quotes, "This long disease, my life," and attributes it to Wordsworth. It's actually a quote from Alexander Pope.
When Drew first gives Miriam a gun, she carries it as she walks to the end of the porch to see shadows moving in the cemetery. As she turns back, she acts as if she is slipping the gun into a pocket of her chiffon dress, although the dress has no pockets. She then walks into the house and does some business, but the gun has disappeared.
Disturbed by a noise in the middle of the night, Miriam gets out of bed and puts a revolver in the pocket of her negligee. Shortly after, a wind storm whirls the gown around her head, something that could never occur if there was actually something as heavy as a gun in her pocket.
(at around 47 mins) The May 6, 1927 Hollisport News has a sub-headline of "Officers in 8 States Seek Mystery Plane". This same secondary headline also appears in newspapers in King of the Underworld (1939), One Against Many (1956), and Mr. Moto gioca d'azzardo (1938).
Charlotte shooting at bulldozer from balcony. Racks and shoulders a shotgun but the sound effect is a bullet ricochet off the dozer.
Sheriff about to grab door knocker when maid opens door before he has a chance to use it. Note that it's still lightly swinging from the previous takes.
In the ballroom dance set in 1927, the women's hairstyles are contemporary with 1964.
Near the beginning, when Charlotte confronts the work crew, in the long shot she says, "I told you to get off my property.", but her mouth isn't moving.
In the opening sequence, the obviously dubbed audio by Bette Davis doesn't match the younger actress's movements on screen: the dialogue is heard as "No, papa", whilst the actress is seen to be saying only "No".
In the beginning where Charlotte is being told that her lover was not going to run away with her, there are two birds in a cage. The birds look like some type of Amazon Parrot, but the sounds they make are more like a parakeet.
When Harry Willis (Cecil Kellaway) arrives in Hollisport by train, the chugging sound of a steam locomotive is heard as the train pulls away. In 1964, passenger trains would have been powered by diesel locomotives, as virtually all US railroads had abandoned steam power ten years earlier.
When Charlotte becomes irate and chases the packers from the house, the camera follows them fleeing from room to room. For a split second, a shadow of the camera or dolly is visible on the near wall as it moves from right to left.
Throughout the film, Charlotte and Miriam keep referring to the "county commissioner". Louisiana is one of two states in the U.S. that's not divided into counties; the other is Alaska.