Madonna has performed "Human Nature" on three of her concert tours, the last being The MDNA Tour of 2012, where it was the subject of controversy when the singer exposed her nipples during a show in Istanbul.
Written and produced by Madonna and Dave Hall, "Human Nature" includes a looping sample from Main Source's 1994 track "What You Need", therefore its writers Shawn McKenzie, Kevin McKenzie and Milo Deering are also credited.
In the United Kingdom, the single entered the chart and peaked at number eight and it also charted within the top ten in Italy.
"Human Nature" is a R&B track where the sound of drums and the sample is heard looping throughout, with Madonna sarcastically asking rhetorical questions based on her real-life actions over the last two years.
"Human Nature" became a moderate hit in the United States, peaking at number 46 on the Billboard Hot 100 and at number two on the Hot Dance Club Play chart.