| Abraham Benrubi | ... | Cyborg / Kilowog / Appa Ali Apsa / Icicle / Solomon Grundy (voice) | |
| Alex Borstein | ... | Wonder Woman / Giganta / Woman (voice) | |
| Nathan Fillion | ... | Green Lantern / Mr. Freeze (voice) | |
| Megan Fox | ... | Lois Lane (voice) | |
| Clare Grant | ... | Cheetah / Ice / Abby Holland (voice) | |
| Seth Green | ... | The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice) | |
| Neil Patrick Harris | ... | Two-Face / Black Manta (voice) | |
| Breckin Meyer | ... | Superman / Mirror Master (voice) | |
| Alfred Molina | ... | Lex Luthor / Firestorm (voice) | |
| Aaron Paul | ... | Mister Banjo / Glenn (voice) | |
| Paul Reubens | ... | The Riddler (voice) | |
| Tom Root | ... | Captain Cold (voice) | |
| Kevin Shinick | ... | Narrator (voice) | |
| Matthew Senreich | ... | The Flash / Chillblaine / Brainiac (voice) | |
| Tara Strong | ... | Harley Quinn / Selena Gomez (voice) | |
| Zeb Wells | ... | Sinestro / B'dg (voice) |
Directed by | |||
| Seth Green | |||
Writing credits(in alphabetical order) | ||
| Matthew Ireland Beans | ||
| Mike Fasolo | ||
| Douglas Goldstein | ||
| Seth Green | ||
| Geoff Johns | ||
| Breckin Meyer | ||
| Tom Root | ||
| Matthew Senreich | ||
| Kevin Shinick | ||
| Zeb Wells | ||
Original Music by | |||
| Shawn Patterson | |||
Cinematography by | |||
| Helder Sun | |||
Film Editing by | |||
| Jenny McKibben | |||
Costume Design by | |||
| Becky Van Cleve | |||
Production Management | |||
| Debi Cohen | .... | post-production supervisor | |
Art Department | |||
| Chelsea Bayouth | .... | junior puppet fabricator | |
| Melissa Bloom | .... | junior puppet fabricator | |
| Dan Field | .... | lead puppet fabricator | |
| Lindsey Gilbert | .... | puppet department coordinator | |
| Lauren Horoszewski | .... | senior puppet fabricator | |
| Teresa Pilar Huarte | .... | model builder | |
| Tom Keiser | .... | lead puppet fabricator | |
| Lizzy Klein | .... | art department intern | |
| Tennessee Reid Norton | .... | head of puppet fabrication | |
| Nikki Rice | .... | senior puppet fabricator | |
| David Waddle | .... | model builder | |
| Christopher Robert Watson | .... | senior molding & casting technician (as Chris '42' Watson) | |
Sound Department | |||
| Timothy J. Borquez | .... | supervising sound editor (uncredited) | |
| Matt Brown | .... | supervising dialogue editor (uncredited) | |
| Krandal Crews | .... | supervising adr editor (uncredited) | |
| Eric Freeman | .... | sound effects editor (uncredited) | |
| Diane Greco | .... | foley artist (uncredited) | |
| Tony Orozco | .... | sound effects editor (uncredited) | |
| Thomas Syslo | .... | supervising sound editor (uncredited) | |
Visual Effects by | |||
| Brian Kokoszka | .... | visual effects artist | |
| Jason Oshman | .... | visual effects intern | |
| Nick Willard | .... | visual effects intern | |
| Kaitlyn Yang | .... | visual effects artist | |
Camera and Electrical Department | |||
| Noah Dille | .... | camera operator (as Noah Dile) | |
| Nicole Emmons | .... | lighting technician | |
Animation Department | |||
| Danielle Bonadona | .... | animation intern | |
| Harry Chaskin | .... | animator | |
| Jeremy Fisher | .... | track reader | |
| Melissa Goodwin | .... | animator | |
| Michael Granberry | .... | animator | |
| Anthony Straus | .... | animator | |
Editorial Department | |||
| Vanara Taing | .... | editor: behind the scenes | |
Music Department | |||
| Steven Tyler | .... | musical performance by | |
Other crew | |||
| Laura Allen | .... | production coordinator | |
| Alan Franzenburg | .... | technical supervisor | |
| Sofia Iffla | .... | assistant to producers | |
| Tom Smith | .... | production assistant (as Tom Smith XXVIII) | |
| Full cast and crew | Company credits | External reviews |
| News articles | IMDb Animation section | IMDb USA section |