The high school photo of Jane Rizzoli found by Frost is Angie Harmon's actual high school picture.
At the reunion, Maura (Sasha Alexander) asks Jane, "Is that your bête noire?" Bête Noire is the title of an episode on NCIS important in Sasha Alexander's character Caitlin Todd's storyline.
In the scene where Jane and Frost are searching Rory's car, they are in the trunk with the ALS and they see a spot that floresces. Jane says that it's blood, but blood does not floresce under UV light. It shows up as a black spot because it absorbs the light, not reflects it. What they saw could have been among other thing semen but definitely not blood.
The reunion is for the Class of '94. Since the episode came out in December 2011, it would either be a 17-year reunion or an 18-year reunion, if going by the school year. This is odd, since most high school reunions are in multiples of 5.
Many of the episode titles are also the names of songs; "Seventeen Ain't So Sweet" is by The Red Jumpsuit Apparatus.