Guardians of the Galaxy (2014)
-Star-Lord appeared in Marvel Preview #4 (January, 1976).
-Rocket Raccoon appeared in Marvel Preview #7 (Summer, 1976).
-Gamora appeared in Strange Tales #180 (June, 1975).
-Drax the Destroyer appeared in Iron Man #55 (February, 1973).
-Groot appeared in in Tales to Astonish #13 (November, 1960).
-Iron Man Three (2013) - Aldrich Killian loses an arm during the battle with Tony Stark.
-Thor: The Dark World (2013) - Loki cuts off Thor's right arm on Svartalfheim.
-Captain America: The Winter Soldier (2014) - Bucky lost his left arm during his fall from the train.
-Guardians of the Galaxy - Gamora cuts off Groot's arms during the group's initial scuffle on Xandar, and Nebula cuts off her own hand to get away from Gamora.
-Avengers: Age of Ultron (2015) - Ultron cuts off the right arm of Ulysses Klaue.