To pitch the show to the Fox network, the creators of the show made a rough three minute demo of the scene where Bob and Linda are grinding the meat and Bob forgets their wedding anniversary. The differences between the demo and the scene in the actual show included very different character designs by Jay Howell, cruder animation and the Belchers were originally a family of cannibals, making burgers out of the flesh of their victims. However, Fox found the idea of cannibalism too dark for an animated sitcom so it was taken out. Although, this lead to the plot of the first episode where the family is accused by the health inspectors of being cannibals.
In the beginning credits, the building to the right of Bob's restaurant changes every episode. The signs for the building in Season 1 in order are: P.F.E.T.A (Meat is Murder), Trot's All Natural Fertilizer, Rent Reduced Crime Scene Special (with police tape around it), Meth I Can Methadone Clinic, Synthesizers! Synthesizers! Synthesizers!, Extra Moist Yog(presumably yogurt), The Horse Renderer, Attempted Crepe French Cooking School, STD-B Gone! Free Clinic, Rocky Refuge Raccoon Sanctuary (referenced in the episode), Bass Drum Emporium (The only 24 hour drum shop in town!), Magnum G.I. Colonoscopies, and Chris's Brisses.
In the pilot demo for the show, the character of Tina Belcher does not appear. Instead, the eldest Belcher child is a son named Dan. Although Dan's appearance is distinct from Tina's, the two characters are voiced identically by Dan Mintz.
An ongoing joke in the series is the fact that the building to the right of Bob's Burgers changes every episode. In Season 2 the building's signs in order are: Uncle Marty's Breast Pumps, Hannibal's Dead Animals Taxidermy, Pookah Pagoda Pookah Shell Warehouse, A Fridge Too Far Used Appliances (Clearance Sale), That's Improv-able Improv Theater (Tickets on Sale Now), Stickers For Men (Now Open), Grindecologist Coffee Shop S (possibly "Supply"), Valley of the Doilies (Sale 50% off), and Yes, Wire Hangers.
The Belcher Kids and their friends attend Wagstaff Middle School, which is named for Professor Quincy Adams Wagstaff, the character played by Groucho Marx in the movie Horse Feathers (1932). Compounding this nod to the Marx Bros. classic, Louise's nemesis Logan attends Huxley High School which takes it's name from Huxley University, over which Professor Wagstaff presided.