When released in the US, the film's original title was changed from 'The Pirates! In an Adventure with Scientists!' to 'The Pirates! Band of Misfits,' with some of the original voice cast replaced with American actors. The Netflix version in the UK and Ireland retain the original cast however use the US title, although the end credits do not credit any actor with their performance.
When Queen Victoria tells the Pirate Captain that "I may have the body of a weak woman" she is quoting from her predecessor Elizabeth I, who made a very similar remark in a famous speech in 1588 when England when threatened with the approach of the Spanish Armada.
When the banished Pirate Captain is trying to sell baby clothes in London, a man is seen walking by, wearing oddly shaped gloves and a bag on his head with a single eye-hole. This is a reference to Joseph Merrick, a.k.a. The Elephant Man, a heavily deformed man, victim of the Proteus Syndrome, who lived in England between around the 1870's and became some kind of national celebrity. When going out in public, Merrick would wear the outfit seen in the movie.
The film is based on Gideon Defoe's novels 'The Pirates! in an Adventure with Scientists' (the Pirates encounter Darwin and Bobo) and 'The Pirates! in an Adventure with Whaling' (the Pirates face off against Cutlass Liz).
During the film, Brendan Gleeson's character Pirate with Gout, has a Blue Peter badge on his hat. This badge was given away on the show Blue Peter (1958), also the name for the show is derived from the flag flown from ships leaving port.