According to Nimród Antal and Robert Rodriguez on the commentary, in the script, the character Cuchillo was described as "a guy who looks like Danny Trejo." When Danny Trejo heard this, he called Robert Rodriguez and said, "hey, I heard there's a guy in the script for 'Predators' who looks just like Danny Trejo and guess what, I look just like Danny Trejo!"
The original script contained cameo appearances by Dutch (Arnold Schwarzenegger) and Michael Harrigan (Danny Glover), the protagonists of the previous Predator films. However, these appearances were ultimately cut out.
Hanzo is missing two fingers on his left hand. The reason that the Yakuza traditionally cut their fingers starting from the left pinkie is that these fingers have a vital role in controlling a Japanese sword, and their loss would significantly impair a duelist. Hanzo must be a formidable swordsman to be able fight in close combat as he did despite the loss of his fingers.
The plant that Edwin (Topher Grace) identifies as "Archaefructus liaoningensis" has actually been extinct for several million years; its origin lies in the Cretaceous Period. Angiosperm fossils were found in China that are believed to be about 125 million years old, making it the world's earliest known flowering plant. In an earlier draft of the script, Edwin highlights the plant's origins, citing it as yet another inexplicable oddity before they finally realize where they are.
Nimród Antal specifically chose Adrien Brody for the main protagonist: "It was a challenge in finding a balance. When we cast Adrien, there were a lot of people going, What? But at the same time, if we cast a Vin Diesel in that role or anyone who is Arnold-esque, we would have been attacked for doing that. So we decided early on to go in a very different direction as far as the casting process, but it turned out fantastic." He also felt the soldiers should be portrayed as wiry tough guys, not burly men like Arnold Schwarzenegger.
The pool with the overhanging rock where the group ends up after the jump from the cliff was found in Texas during location scouting. However, when the scene was shot months later, the temperature had dropped considerably, and water had started to flow from the rock. Not only did the actors have to be submerged in freezing cold water, the noise from the waterfall made the recording of usable dialogue impossible.
According to Robert Rodriguez on the DVD commentary, Walton Goggins had been cast in the role of Stans, but Rodriguez felt the character was written far too much like the character Hudson in Aliens (1986). So Rodriguez had the part re-written and asked director Nimród Antal to re-cast the role. But Antal insisted that Goggins could still do the role and scheduled a meeting between Goggins, Rodriguez and himself. Within a very short period of time, Rodriguez was convinced that Walton was still right for the part.
The film's basic plot was conceived in 1994, when Robert Rodriguez was working on Desperado (1995). He presented a draft of the script to 20th Century Fox, but they turned it down because the budget required was too large. 15 years later, the studio decided to follow through with his script; in the end, an updated version of his script.
According to Robert Rodriguez, the title of "Predators" serves as a double-entendre, describing the alien hunters as well as the ensemble human characters they target: "They could very well kill each other off even if there were no Predators!"
The song that plays over the credits is Little Richard's "Long Tall Sally" which was listened to by the main characters of the first Predator (1987) during the scene where they're dropped into the jungle for their mission.
Royce, Isabelle, Nikolai and Noland are the only people to say their names in the film. Stans, Mombasa, Cuchillo, Edwin and Hanzo's names are never revealed (although Stans identifies himself in a deleted scene). The DVD captions identify all characters.
The man whose parachute fails to open is wearing pixel-gray Army Combat Uniform fatigues, meaning he was most likely a soldier in the United States Army. The same goes for the unidentified man found later with his chest ripped open: Nikolai reads from the man's notebook that he was supposed to be stationed in Afghanistan.
Isabelle uses a Blaser R93 sniper rifle fitted with an Elcan DigitalHunter scope. The official sniper rifle for active-duty Israeli Defense Forces personnel is actually the H-S Precision HTR, and it achieved that designation in May 2010, only a month before this movie was released.
In Decloaking the Invisible: Alien Terrain (2010), it can plainly be seen that stunt performers were used for the cliff fall scene, in most of the shots, if not all of them, that were actually used in the movie. This is especially true for Edwin's fall. This was done by a professional, and he still accidentally landed mostly on his head.
Nikolai was originally supposed to be armed with a Russian GShG-7.62 four-barreled rotary machinegun, as it would be more fitting for a Russian spetsnaz operator. The production crew was unable to obtain one, so they used an American M134 minigun instead.
Stans is a death row inmate in California's San Quentin Correctional Facility. All death row inmates can receive a pardon or stay of execution by the state's governor. At the time this film was made (and presumably the time the movie takes place), California's governor was Arnold Schwarzenegger, the lead star of the original Predator (1987) film.
The Insectoid Alien that appears around the 50 minute mark is an allusion to the original design of The Predator from the First film when it was played by Jean-Claude Van Damm. The creature was redesigned after Van Damm's departure to the current design.
Even though this installment in the "Predator" franchise explicitly wanted to part with the crossover AvP story arc, it does contain at least three nods to the "Alien" franchise: - 1) at one point, while (obviously, given the movie's universe) facing near-certain death, one character tells another "If the time comes, I'll do us both", a reference to Hicks' almost identical line in Aliens (1986), 2) when the group finds the body of an earlier victim of the antagonists, he has a large hole in his chest with the ribs bent outwards (probably from a Predator energy blast shot from behind), referencing the way xenomorph young emerge from their host and the wound found on the "space jockey" in Alien (1979), also Nikolai references Billy's dialogue from Predator (1987), "he set up position here, he was shooting in all directions" 3) as soon as Royce recovers from his parachute landing; as he looks around a music motif from Aliens can be heard and when the group enters the Predators camp there's a brief view of an Alien skull on the ground. Additionally, when realizing that the Classic Predators may be helpful against the Super Predators, Royce mentions 'my enemy's enemy', which references the 'enemy of the enemy is my friend' concept from AVP: Alien vs. Predator (2004).