- Brains and Wheelie watch Star Trek: Amok Time (1967), noting "I've seen this one. It's the one where Spock goes nuts."
- Sam describes Gould's building as "the Starship Enterprise."
- when the Autobots are leaving, Bumblebee's farewell to Sam includes the words "My friend", sampled from Star Trek II: The Wrath of Khan (1982) ("You are...my friend. I have been and always shall be yours.").
- Sentinel Prime says "The needs of the many outweigh the needs of the few", a maxim of Spock.