When writing her name on the sign up sheet for the glee club, Tina adds extra Ts at the beginning to mimic the stutter that she often speaks with. When Rachel comes to write her name underneath Tina's the extra Ts are gone and Tina is written properly.
After Finn claimed his mother was having his prostate removed while actually going to Glee, Puck confronts him and claims "Chicks don't have prostates, I looked it up." However in a 2002 medical research study, it was found they do.
When we hear the narration by Rachel in the beginning of the episode, she states that she tries to post on MySpace everyday to keep her talent alive and growing. However, when the Cheerios are watching Rachel's cover of "On My Own", in the box that says "Similar Videos", it states that there are no other videos from Rachel.