Harley says he would like to add retro-reflective panels to Iron Man to make him stealthy. This would have the opposite effect and greatly increase Iron Man's detectability since that is the very purpose of retro-reflection.
Radical differences between altitudes when Iron Man is saving the airline passengers. At one point Jarvis calls out "18,000" feet and then only eight seconds later he calls out "10,000" feet. Nothing falls at 1,000 miles per hour from natural gravity. Then Jarvis calls out the last 9,400 feet in twenty seconds, nearly three times longer than it took the first distance.
When Harley first meets Tony in his garage, he realizes who Tony is after seeing the Mark 42 suit - he acknowledges this by handing Tony the newspaper with the article about his Malibu home being blown up. So why did Harley ask Tony his name a few minutes later ("Harley. And you're...?")?
When Tony and Rhodey are at the docks and taking cover, Tony asks for more ammo from his pistol, even though Rhodey tells him their bullets are not compatible, which Tony insists that they are. While Tony may not have any military training like Rhodey does, he is a notorious former weapons designer, and should know that certain pistols can only use certain caliber bullets.
When Happy is in the ICU the nurse adjusts the roller clamp to the IV fluid. A pump controls the flow; adjusting the roller clamp would do nothing except shut off the medication, causing the machine to call for attention.
Hair lost or burnt off by those injected with Extremis grows back along with missing body parts. While hair is living within the follicle, once it's outside the skin, it's dead and cannot be regenerated in its current state. (As long as the follicle is not damaged, it can be regrown, but styling would not regenerate.)
When Tony is zip-tied to the bed, he asks one of the guards the distance between Miami and Tennessee. The guard says 832 miles, which is correct for some parts of Tennessee. This means Tony's armor is ~832 miles away. Even assuming his armor travels at 1000 miles/hour, it would take his armor almost an hour to get to him, rather than the few minutes shown in the movie. Tony's armor would have to travel at almost 10,000 miles/hour to get to Tony in 5 minutes. The current airspeed record for a plane is only 2,193 miles/hour. Tony's armor, at the current airspeed record, would still take 23 minutes to get to him.
When Tony is observing the map of the U.S. in the wake of the Mandarin's attack on the Chinese Theater, Jarvis brings up the map of all heat signatures in the country. Tony then asks Jarvis to take away everywhere there's been a Mandarin attack. 20-30 heat signatures disappear from the map. Earlier in the movie, Rhodey tells Tony that there have been 9 total attacks, and 3 that the public know about. Where did all the heat signatures come from?