The producers have stated that this film is really just the middle part of a planned trilogy. The next chapter would be a prequel to the events shown in this film and is tentatively titled "Repo!: The Beginning". No time frame has been given for when production could start on the next movie.
Anthony Stewart Head was chosen for his role as Nathan Wallace after Darren Bousman had heard him sing in the Buffy the Vampire Slayer 6th season ep.#7 which was titled "Once More with Feeling". Anthony Head had sung previously in the Buffy the Vampire Slayer series during it's 4th season as well. Anthony Stewart Head has had several other singing credits to his name as well- one song in particular that can be noted is his rendition of the song Sweet Transvestite.
Darren Lynn Bousman originally refused to hold an audition for Paris Hilton. He did not think she would be capable, and feared media backlash or accusations of stunt casting. When he finally caved in, Paris came to the audition dressed "perfectly for the part," and "rocked" the sound booth audition. After the audition, he was convinced she should play the role.
The theatrical version of "Repo! The Genetic Opera" has several songs that were on the Original Motion Picture Soundtrack cut out of the actual movie. Those songs include "Can't Get It Up If The Girl Is Breathing" and "Needle Through A Bug", both were cut for pacing reasons. They are Included as deleted scenes on the DVD and Blu-ray.
The song "Genetic Repoman" was not originally going to be filmed, but when the producers were not around, Darren Lynn Bousman quickly filmed Terrance Zdunich singing. The repossession part of the song was shot 3 months later.
In the first scene on the cemetery, when Shilo hides behind the gravestone (which she previously captured an insect on), we can see the name Peter Block on the stone for a moment. Peter Block is the producer of the movie.
Many people believe the song 'Largo's Little Helpers' is the song with Luigi and his gophers but in reality it is a GeneCo advertisement featuring Orphans talking about Rotti Largo. The song with Luigi and his gophers is really a continuation of the song 'Genterns'.
Rotti was originally supposed to cough up blood onto his handkerchief after Amber reveals her botched surgery face to him, but actor Paul Sorvino refused to cough up blood. As a result, the handkerchief remains clean during the scene.