The televisions in the Buy More store will always display images or footage that will relate to what is occurring within the episode. For example, in Chuck VS the Helicopter, the TVs displays footage of helicopters.
During an interview with The A.V. Club, Vik Sahay said that he first auditioned for the role of Morgan, and when the auditions came down to just himself and Joshua Gomez, and Gomez got it, the producers instead offered Sahay the then-smaller role of Lester. Lester has since become a more prominent character than he was in the pilot.
In a 2013 National Public Radio interview, Zachary Levi said that he was almost in the Broadway musical production of "Young Frankenstein," but he had to give up the opportunity because the pilot for "Chuck" got picked up as a series.
In the episode Chuck: Chuck Versus Santa Claus (2008) there are several references to the movie Die Hard (1988). The LAPD officer in charge is Al Powell, (and played by the same actor, Reginald VelJohnson), who also eats Twinkies. He responds to the FBI agent who asks "Who's in charge here?" just like in the movie. Al Powell also calls his brother, the store manager, Mike Powell, and uses a line from the film "How Ya holding up in there, partner?"
Natalie Martinez was to join the cast after the pilot episode as Kayla Hart, a love interest for Chuck. Martinez appeared in the cast promotional pictures but her character was written out before she could appear on the show.
A good many references are made to the movie Spies Like Us (1985). The bug used in several episodes is called a GLG-20, which is the designation given to undercover operatives in the movie. Emmett Millbarge is named after the two main characters in the movie, first name taken from Chevy Chase's Emmett Fitzhume, and last name taken from Dan Aykroyd's Austin Millbarge; this is also the order they are introduced in the film. In an episode, Chuck poses as a doctor and references a scene from the movie where doctors go through greeting each other by saying "Doctor, Doctor". Chevy Chase has a guest role in the show. In the movie, one of the actors playing the Tadzhik Highway Patrolman has the name Costa Gavras and the name of the communist state featured in "Chuck Versus the Angel of Death" has the name Costa Gravas. Also while Chuck and Sarah are watching TV, Spies Like Us comes on, and Sarah comments "not again".
In Chuck: Chuck Versus the First Bank of Evil (2011) there are actually two references to the popular TV show Lost (2004). The first is when Vivian receives the bank card at Volkoff Industries in which the card displays the famous numbers from Lost (2004) 4815162342. The second reference is at the National Bank of Macau, Guillermo Chan, the head of the bank is played by François Chau, who also played Dr. Pierre Chang from the Dharma Initiative in Lost (2004).
At the close of the second season, declined ratings and stiff competition from the other networks had indicated that Chuck would be cancelled. But due to petitioning from its loyal fan base specifically aimed at one of its sponsors (Subway) the show was renewed for a third season in an unprecedented partnership between NBC and the sandwich chain helping to cover the production costs. Chuck continued to face cancellation for its subsequent seasons with its fifth season becoming its final season beginning October 28, 2011 and ending on January 27, 2012 with a 2 hour finale.
When Dolph Lundgren appears Chuck: Chuck Versus the Anniversary (2010) he makes several references to his character Ivan Drago in Rocky IV (1985). He does so when interrogating John Casey telling him, "I must break you," as well as threatening the team saying, "If they die, they die."
In Chuck: Chuck Versus the Helicopter (2007), Chuck is listing a series of things he knows from the intersect. One of them is a reference to Lost (2004) when he says "Oceanic Flight 815 was shot down by a surface to air...."