This episode features the first appearance of The Chameleon in any Spider-Man cartoon. In the original "Spider-Man (1967)" cartoon The Chameleon was not allowed to appear after making an appearance with Kraven the Hunter on an Iron Man segment of "The Marvel Super Heroes" (1966) and was replaced with renowned actor and master of impersonation named Charles Cameo.
Spider-Man's belt light "spider-signal" from the comics is used for the first time outside of the comics in this episode.
The Avengers have a cameo appearance on TV. The Avengers appearing were: the Scarlet Witch, Captain America, Iron Man, Thor and Vision.