The Lieutenant Commander whom Ro refers to (her instructor at Starfleet Advanced Tactical Training who joined the Maquis) was supposed to be a subtle nod toward Chakotay. But this intention was later contradicted in Star Trek: Voyager: In the Flesh (1998), where we learn that Chakotay resigned his Starfleet commission in 2368, two years before Ro's time on the course.
We see an Indian on one of the Maquis ships. It is possible he is from Dorvan V, the colony that Starfleet handed over to the Cardassians in Star Trek: The Next Generation: Journey's End (1994). Which may be why he joined the Maquis.
The penultimate episode of the show. To mark the occasion, TV Guide presented a special retrospective issue devoted to the long-running show, which had been on the air for seven years and was now about to end.
The bird sculptures seen in the conference room of the Maquis previously appeared in Marla Aster's house in the third season episode "The Bonding" and in the reception area at Arkaria Base in the sixth season episode "Starship Mine".
The trivia items below may give away important plot points.
Ro's departure to the Maquis was intended to set her up as a main character in Star Trek: Voyager (1995), however Michelle Forbes was unwilling to commit to a series, so Roxann Dawson, playing the character B'Elanna Torres, took her place.
Michelle Forbes makes her final appearance as Ro Laren. This was her only episode as a Lieutenant after being an Ensign for two years. She defects to the Maquis, ending her Starfleet career for the second time.