This is the final appearance of Ed and Ein in the series.
At the end where they usually say, "See you, Space Cowboy," they say, "See you cowgirl, someday, somewhere!"
Edward's real name is Francoise Applederry.
In Session 24 "Hard Luck Woman" Ed's father Mr. Appledelhi calls Ed Francoise. However since Ed's father is bad remembering names and can't even remember if Ed's a boy or a girl this might not actually be true.