When Chase works on the crossword puzzle, the close-up reveals CRETINISM already written in. The next scene, however, has him asking Foreman to help him guess that word.
House checks out of the clinic at 4:03pm but in the next scene the clock on House's desk changes from 3:27 to 3:28.
While taking a retinal biopsy, the probe is shown passing through the cornea (i.e. front of the eye through pupil). In this case, the probe passes through an optically clear medium important for refraction, namely the cornea and lens, which would be damaged if done this way. To avoid this, the probe usually enters in sideways, through the sclera (white of the eye), so that the probe can directly pass behind the lens without touching the it.
House gives the cups with the parents' DNA to Cameron and Foreman in plastic bags. Plastic can make DNA mutate. He should have put the cups in paper bags to make sure that the DNA would be unchanged.
When Chase notices the "bowing" of the corpus callosum the camera pans across some sections of the patient's brain MRI. None of the sections shown actually capture the corpus callosum.
After analyzing the patient's MRI with the team, House orders a "radionucleotide" cisternogram, which is a malapropism for the intended term "radionuclide."
During the lacrosse game at the end when the camera pans over the cheering crowd, you can clearly see many of them are dummies dressed up with clothing and wigs. This is a common trick used in television, used here to make the bleachers seem filled up, when in fact there are far fewer extras playing the fans watching the game.
Cameron (and all the young female staff of House's team throughout the series) wears stiletto heeled shoes, which is extremely unlikely on an active hospital team.
In the lacrosse match in the opening moments there is a flipped shot and the name TARTANS was displayed mirrored.
About 30 second into the episode, as the Lacrosse player is running, at one point for a few seconds, the video is horizontally reversed as can be seen by the uniforms being a mirror image of the wording they should be.